Morphine Potentiates Dysbiotic Microbial and Metabolic Shifts in Acute SIV Infection.
Morphine Potentiates Dysbiotic Microbial and Metabolic Shifts in Acute SIV Infection.
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DOI：
10.1007/s11481-018-9805-6
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发表时间：
2019-06
期刊：
影响因子：
--
通讯作者：
Buch S
中科院分区：
文献类型：
--
作者：
Sindberg GM;Callen SE;Banerjee S;Meng J;Hale VL;Hegde R;Cheney PD;Villinger F;Roy S;Buch S
人类免疫缺陷病毒（HIV）的发病机制与微生物易位密切相关，微生物易位被认为是炎症和HIV复制的驱动因素。阿片类药物已被证明会加重这种症状，导致艾滋病毒感染更快地发展为获得性免疫缺陷综合征（艾滋病）。艾滋病毒和阿片类药物的相互作用尚未在艾滋病毒的早期阶段进行研究，特别是在肠道微生物组中，其中变化可能先于易位事件。这项研究通过检查猴免疫缺陷病毒（SIV）感染的灵长类动物在21天或更短的时间内独立地和在阿片类药物使用的背景下模拟早期HIV感染。通过质谱法分析粪便样品的微生物种群的16 S分析以及代谢产物谱。我们的研究结果表明，在检查的时间点，SIV治疗的动物的变化是微小的，然而，用吗啡和SIV治疗的动物的微生物群落和代谢谱有显着的变化。这发生在一个时间无关的方式与吗啡，无论多久的动物有吗啡在其系统。在全球范围内，观察到的变化支持这些动物早期发生微生物生态失调，这可能有助于在SIV/HIV发病机制中观察到的易位事件。此外，代谢变化可预测特定治疗组，可进一步开发为诊断工具或未来干预目标，以克服和减缓艾滋病毒感染向艾滋病的进展。
Human Immunodeficiency Virus (HIV) pathogenesis has been closely linked with microbial translocation, which is believed to drive inflammation and HIV replication. Opioid drugs have been shown to worsen this symptom, leading to a faster progression of HIV infection to Acquired Immunodeficiency Syndrome (AIDS). The interaction of HIV and opioid drugs has not been studied at early stages of HIV, particularly in the gut microbiome where changes may precede translocation events. This study modeled early HIV infection by examining Simian Immunodeficiency Virus (SIV)-infected primates at 21 days or less both independently and in the context of opioid use. Fecal samples were analyzed both for 16S analysis of microbial populations as well as metabolite profiles via mass spectrometry. Our results indicate that changes are minor in SIV treated animals in the time points examined, however animals treated with morphine and SIV had significant changes in their microbial communities and metabolic profiles. This occurred in a time-independent fashion with morphine regardless of how long the animal had morphine in its system. Globally, the observed changes support that microbial dysbiosis is occurring in these animals at an early time, which likely contributes to the translocation events observed later in SIV/HIV pathogenesis. Additionally, metabolic changes were predictive of specific treatment groups, which could be further developed as a diagnostic tool or future intervention target to overcome and slow the progression of HIV infection to AIDS.
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DOI：
10.1097/qad.0b013e32832d3b85
发表时间：
2009-09-10
期刊：
AIDS (London, England)
影响因子：
--
作者：
Grunfeld C;Delaney JA;Wanke C;Currier JS;Scherzer R;Biggs ML;Tien PC;Shlipak MG;Sidney S;Polak JF;O'Leary D;Bacchetti P;Kronmal RA
通讯作者：
Kronmal RA
影响因子：
4.6
作者：
Banerjee, Santanu;Meng, Jingjing;Das, Subhas;Krishnan, Anitha;Haworth, Justin;Charboneau, Richard;Zeng, Yan;Ramakrishnan, Sundaram;Roy, Sabita
通讯作者：
Roy, Sabita
影响因子：
3.7
作者：
Gómez-Hurtado I;Santacruz A;Peiró G;Zapater P;Gutiérrez A;Pérez-Mateo M;Sanz Y;Francés R
通讯作者：
Francés R
影响因子：
3.7
作者：
Bi S;Meng J;McLean S;Wu W;Ni X;Ye J
通讯作者：
Ye J
影响因子：
6.2
作者：
Bandaru, Veera Venkata Ratnam;Patel, Neha;Haughey, Norman J.
通讯作者：
Haughey, Norman J.