Abnormal surfactant metabolism and function in preterm ventilated baboons.
Abnormal surfactant metabolism and function in preterm ventilated baboons.
复制标题
早产通气狒狒的表面活性剂代谢和功能异常。
DOI：
10.1164/ajrccm.158.6.9804128
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发表时间：
1998
影响因子：
24.7
通讯作者：
Ikegami,M
中科院分区：
文献类型：
--
作者：
Seidner,SR;Jobe,AH;Coalson,JJ;Ikegami,M
我们评估了肺表面活性物质的代谢和功能，以及产前糖皮质激素对早产儿的影响。在妊娠125±2d(足月为184d)分娩前48h和24h，将怀孕的狒狒随机分为生理盐水(对照组)和倍他米松(β)6 mg组。用[~(14)C]二棕榈酰磷脂酰胆碱标记的表面活性物质处理新生狒狒并进行6d的呼吸，6只对照组和6只倍他米松处理组的肺功能相似。14C饱和磷脂酰胆碱(Sat PC)在肺泡冲洗液中的回收率分别为4.8%和3.6%，在全肺中的回收率分别为15.4%和17.7%。Sat PC的肺泡池和总肺池大小分别约为2 3和1 90μ/kg。从第5天到第6天，早产狒狒分泌了8.7%(对照)和6.5%(β)的~3H-棕榈酸标记的新生合成的Sat PC。这些早产狒狒具有较高的Sat PC合成和净组织积累率，而Sat PC的分泌量较低。来自早产狒狒的大聚集表面活性物质组分具有高的最低表面张力，当用于治疗缺乏表面活性物质的早产兔时，效果不如来自新生或成年狒狒的表面活性物质。早产儿的呼吸与肺表面活性物质的功能和代谢异常有关，这些异常不会被产前糖皮质激素改变。
We evaluated surfactant metabolism and function and the effects of antenatal glucocorticoids in very preterm baboons. Pregnant baboons were randomized to receive saline (controls) or 6 mg betamethasone (beta) 48 and 24 h before delivery at 125 ± 2 d gestation (term is 184 d). The newborn baboons were treated with [14C]dipalmitoylphosphatidylcholine-labeled surfactant and ventilated for 6 d. Lung function for six control and six betamethasone-treated animals was similar. Recoveries of14C-saturated phosphatidylcholine (Sat PC) were similar: 4.8% (control) and 3.6% (beta) in alveolar wash and 15.4% (control) and 17.7% (beta) in total lungs. Alveolar and total lung pool sizes of Sat PC were about 23 and 190 μ mol/kg, respectively. The preterm baboons secreted 8.7% (control) and 6.5% (beta) of de novo synthesized Sat PC labeled with3H-palmitate from Day 5 to Day 6. These preterm baboons had high estimated Sat PC synthetic and net tissue accumulation rates but low secretion of Sat PC. The large aggregate surfactant fractions from the preterm baboons had high minimal surface tensions and were less effective when used to treat surfactant-deficient preterm rabbits than surfactant from newborn or adult baboons. Ventilation of the preterm baboon was associated with surfactant functional and metabolic abnormalities that were not altered by antenatal glucocorticoids.