Gold nanoparticles-functionalized microorganisms assisted construction of immunobiosensor for sensitive detection of ochratoxin A in food samples
Gold nanoparticles-functionalized microorganisms assisted construction of immunobiosensor for sensitive detection of ochratoxin A in food samples
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DOI：
10.1016/j.snb.2019.126969
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发表时间：
2019-11-15
影响因子：
8.4
通讯作者：
Wang, Li
中科院分区：
文献类型：
--
作者：
Bu, Tong;Zhang, Meng;Wang, Li
以微生物为还原剂和载体，建立了一种简便、灵敏的赭曲霉毒素A（OTA）免疫层析检测方法。本工作通过酵母菌/乳酸菌（LAB）与HAuCl 4的生物吸附和随后的生物还原反应合成了微生物负载金纳米颗粒（MO@AuNPs）复合材料。研究了MO@AuNPs的合成条件（pH和反应时间）和主要参数（形貌、粒径和官能团）。由于MO@AuNPs的大尺寸（约2 μ m）和高吸附能力，标记的抗体很少，导致伊卡的检测限较低。优化后，基于MO@ AuNPs的免疫分析法检测OTA的线性范围为0.1 ~ 1.6 ng/mL，检测限（vLOD）为0.1 ng/mL，比传统的AuNPs-ICA低近8倍。同时，该策略对OTA对其他真菌毒素也具有很高的特异性。通过对大米、玉米、生姜和绿色豆类样品中OTA的检测，验证了该平台的适用性。结果表明，这些创新的传感元件是有前途的替代品，并为生物检测带来了创新的灵感。
A simple and novel immunochromatographic assay (ICA) was developed for specific and sensitive determination of ochratoxin A (OTA) employing microorganism (MO) as reducers and carriers. In this work, microorganism loaded gold nanoparticles (MO@AuNPs) composites were synthesized by reacting Yeast/Lactobacillus (LAB) with HAuCl4 via biosorption and subsequent bioreduction. The synthesis conditions (pH and reaction time) and main parameters (morphology, diameter and functional groups) of MO@AuNPs were investigated. Owing to the large sizes (about 2 mu m) and high adsorption abilities of MO@AuNPs, the few antibodies were labeled, resulting a lower detection limit for ICA. After optimization, the linear detection of OTA using MO@AuNPs-based immunoassay ranged from 0.1 to 1.6 ng/mL, and the visual limit of detection (vLOD) was 0.1 ng/mL, which was nearly 8-fold lower than traditional AuNPs-ICA. Meanwhile, the proposed strategy also possessed high specificity for OTA against other mycotoxins. The applicability of the platform was successfully assessed through the detection of OTA in rice, corn, ginger and green bean samples. The results indicated that these creative sensing elements are promising alternatives and bring an innovative inspiration for biological detections.