Unbiased analyses of ITS folding motifs in a taxonomically confusing lineage: Anagnostidinema visiae sp. nov. ( cyanobacteria )
Unbiased analyses of ITS folding motifs in a taxonomically confusing lineage: Anagnostidinema visiae sp. nov. ( cyanobacteria )
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对分类学上令人困惑的谱系中 ITS 折叠基序的公正分析：Anagnostidinema visiae sp。
DOI：
10.1111/jpy.13337
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发表时间：
2023
影响因子：
2.9
通讯作者：
Casamatta, Dale A.
中科院分区：
文献类型：
--
作者：
McGovern, Callahan A.;Norwich, Alyson R.;Thomas, Aimee L.;Hamsher, Sarah E.;Biddanda, Bopaiah A.;Weinke, Anthony D.;Casamatta, Dale A.
蓝细菌是一种多样的原核光合生物，存在于几乎所有已知的生态系统中。最近在世界各地的调查已经恢复了大量的新的生物多样性在很少采样的栖息地。一个重要的遗传学特征，16 S-23 S ITS rDNA区域的二级折叠结构，允许建立新物种的前所未有的能力。然而，两个问题出现了：这个功能是作为信息的建议，我们如何最好地利用这些功能？美国休伦湖（Lake Huron）的缺氧、富硫地下水淹没的天坑含有以产氧和缺氧蓝藻为主的微生物垫。我们试图记录一些这种独特的蓝藻多样性。使用基于培养的调查，我们回收了45个菌株，其中23个使用16 S-23 S rDNA序列，ITS折叠模式，生态学和形态学进行了分析。由于缺乏形态上的不连续性和模糊的16 S rDNA基因序列差异，ITS折叠模式有效地表达了隐藏的生物多样性。然而，如果我们没有折叠所有来自菌株的可用基序，包括那些具有高度相似的16 S rDNA基因序列的基序，我们可能会错过这些特征。如果我们仅仅依靠形态学或16 S rDNA基因数据，那么我们很可能会错过Anagnostidinema的多样性。因此，为了避免构象的基础上，这是潜在的共同时，采用ITS结构，我们主张聚类菌株的基础上ITS rDNA区域模式独立和比较他们回到16 S rDNA基因同源性。使用全证据的方法，我们建立了一个新的分类单元，根据国际藻类，真菌和植物命名规则：Anagnostidinemaericae。
Cyanobacteria are diverse prokaryotic, photosynthetic organisms present in nearly every known ecosystem. Recent investigations around the world have recovered vast amounts of novel biodiversity in seldom sampled habitats. One phylogenetically significant character, the secondary folding structures of the 16S–23S ITS rDNA region, has allowed an unprecedented capacity to erect new species. However, two questions arise: Is this feature as informative as is proposed, and how do we best employ these features? Submerged sinkholes with oxygen‐poor, sulfur‐rich ground water in Lake Huron (USA) contain microbial mats dominated by both oxygenic and anoxygenic cyanobacteria. We sought to document some of this unique cyanobacterial diversity. Using culture‐based investigations, we recovered 45 strains, of which 23 were analyzed employing 16S–23S rDNA sequences, ITS folding patterns, ecology, and morphology. With scant morphological discontinuities and nebulous 16S rDNA gene sequence divergence, ITS folding patterns were effective at articulating cryptic biodiversity. However, we would have missed these features had we not folded all the available motifs from the strains, including those with highly similar 16S rDNA gene sequences. If we had relied solely on morphological or 16S rDNA gene data, then we might well have missed the diversity ofAnagnostidinema. Thus, in order to avoid conformation basis, which is potentially common when employing ITS structures, we advocate clustering strains based on ITS rDNA region patterns independently and comparing them back to 16S rDNA gene phylogenies. Using a total evidence approach, we erected a new taxon according to the International Code of Nomenclature for Algae, Fungi, and Plants:Anagnostidinema visiae.