Role of AMPK throughout meiotic maturation in the mouse oocyte: evidence for promotion of polar body formation and suppression of premature activation.
Role of AMPK throughout meiotic maturation in the mouse oocyte: evidence for promotion of polar body formation and suppression of premature activation.
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DOI：
10.1002/mrd.21229
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发表时间：
2010-10
影响因子：
2.5
通讯作者：
Davis, Christopher C.
中科院分区：
文献类型：
--
作者：
Downs, Stephen M.;Ya, Ru;Davis, Christopher C.
本研究旨在探讨AMPK对小鼠卵母细胞从萌发泡期到中期II减数分裂的调控作用。暴露小鼠卵丘细胞封闭的卵母细胞（CEO）和裸露的卵母细胞（DO）在体外自发成熟的AMPK激活剂导致增加的速度和频率的极体形成。AMPK的抑制剂具有相反的抑制作用。此外，AMPK抑制剂化合物C（Cmpd C）增加卵母细胞活化的频率。AMPK激活剂AICAR的刺激作用和Cmpd C的抑制作用如果暴露延迟则减弱，表明AMPK对极体形成的早期作用。自发和Cmpd C诱导的激活频率在CEO的激素引发的时间增加，减少和AMPK刺激消除激活反应。用抗AMPK抗体对卵母细胞进行免疫染色，结果显示在成熟过程中AMPK活性与染色质、纺锤体极和中间体有关。AMPK α1催化亚基的免疫定位显示与浓缩的染色质和减数分裂纺锤体相关，但不在纺锤体极或中间体中; α2仅在整个卵母细胞中弥漫染色。这些数据表明，AMPK参与整个成熟过程的调节能力，并有助于促进减数分裂的完成，同时抑制过早激活。
This study was conducted to assess the role of AMPK in regulating meiosis in mouse oocytes from the germinal vesicle stage to metaphase II. Exposure of mouse cumulus cell-enclosed oocytes (CEO) and denuded oocytes (DO) during spontaneous maturation in vitro to AMPK-activating agents resulted in augmentation of the rate and frequency of polar body formation. Inhibitors of AMPK had an opposite, inhibitory effect. In addition, the AMPK inhibitor, compound C (Cmpd C) increased the frequency of oocyte activation. The stimulatory action of the AMPK-activating agent, AICAR, and the inhibitory action of Cmpd C were diminished if exposure was delayed, indicating an early action of AMPK on polar body formation. The frequency of spontaneous and Cmpd C-induced activation in CEO was reduced as the period of hormonal priming was increased, and AMPK stimulation eliminated the activation response. Immunostaining of oocytes with antibody to active AMPK revealed an association of active kinase with chromatin, spindle poles and midbody during maturation. Immunolocalization of the α1 catalytic subunit of AMPK showed an association with condensed chromatin and the meiotic spindle, but not in the spindle poles or midbody; α2 stained only diffusely throughout the oocyte. These data suggest that AMPK is involved in a regulatory capacity throughout maturation and helps promote the completion of meiosis while suppressing premature activation.
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DOI：
10.1016/0304-4165(96)00021-9
发表时间：
1996-06-04
影响因子：
3
作者：
Henin, N;Vincent, MF;VandenBerghe, G
通讯作者：
VandenBerghe, G
影响因子：
3.8
作者：
Fissore, RA;Kurokawa, M;Smyth, J
通讯作者：
Smyth, J
影响因子：
2.7
作者：
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通讯作者：
Downs, Stephen M.
影响因子：
2.5
作者：
Bilodeau-Goeseels, Sylvie;Sasseville, Maxime;Richard, Francois J.
通讯作者：
Richard, Francois J.
影响因子：
3.7
作者：
Baumann, Philipp;Mandl-Weber, Sonja;Schmidmaier, Ralf
通讯作者：
Schmidmaier, Ralf