Existence and Distinction of Acid-Evoked Currents in Rat Astrocytes
Existence and Distinction of Acid-Evoked Currents in Rat Astrocytes
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DOI：
10.1002/glia.21017
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发表时间：
2010-09-01
期刊：
影响因子：
6.2
通讯作者：
Chen, Jian-Guo
中科院分区：
文献类型：
--
作者：
Huang, Chao;Hu, Zhuang-Li;Chen, Jian-Guo
星形胶质细胞是支持和/或保护邻近神经元免受病理损害的重要结构。虽然普遍认为谷氨酸受体介导了大多数星形胶质细胞的作用，但酸诱发电流在这方面的作用最近引起了人们的注意。在这里，我们确定了酸敏感离子通道（ASIC）和瞬时受体电位香草酸1型（TRPV 1）在星形胶质细胞的存在和特点。在酸性溶液（pH 6.0）作用下，培养的大鼠星形胶质细胞可记录到两种类型的电流。10%的星形胶质细胞呈现瞬时电流，其余90%的星形胶质细胞呈现持续电流，分别与ASIC和TRPV 1电流的特征一致。免疫印迹和免疫荧光证实了ASIC 1，ASIC 2a，ASIC 3和TRPV 1在培养和原位星形胶质细胞中的表达。与神经元中ASICs主要分布在细胞膜/细胞质中不同，星形胶质细胞中ASICs主要表达在细胞核中。TRPV 1在培养的星形胶质细胞中对Na+具有更强的渗透性，这与典型的神经元TRPV 1主要对Ca 2+具有渗透性不同。本研究证实了大鼠星形胶质细胞存在两种酸诱发电流，为进一步了解星形胶质细胞配体门控离子通道的功能提供了新的思路。(C)2010 Wiley-Liss，Inc.
Astrocytes are vital structures that support and/or protect neighboring neurons from pathology. Although it is generally accepted that glutamate receptors mediate most astrocyte effects, acid-evoked currents have recently attracted attention for their role in this regard. Here, we identified the existence and characteristics of acid-sensing ion channels (ASICs) and the transient receptor potential vanilloid type 1 (TRPV1) in astrocytes. There were two types of currents recorded under the application of acidic solution (pH 6.0) in cultured rat astrocytes. Transient currents were exhibited by 10% of the astrocytes, and sustained currents were exhibited by the other 90%, consistent with the features of ASIC and TRPV1 currents, respectively. Western blotting and immunofluorescence confirmed the expression of ASIC1, ASIC2a, ASIC3, and TRPV1 in cultured and in situ astrocytes. Unlike the ASICs expressed in neurons, which were mainly distributed in the cell membrane/cytoplasm, most of the ASICs in astrocytes were expressed in the nucleus. TRPV1 was more permeable to Na+ in cultured astrocytes, which differed from the typical neuronal TRPV1 that was mainly permeable to Ca2+. This study demonstrates that there are two kinds of acid-evoked currents in rat astrocytes, which may provide a new understanding about the functions of ligand-gated ion channels in astrocytes. (C) 2010 Wiley-Liss, Inc.