PURIFICATION OF PROFILIN FROM SACCHAROMYCES-CEREVISIAE AND ANALYSIS OF PROFILIN-DEFICIENT CELLS
PURIFICATION OF PROFILIN FROM SACCHAROMYCES-CEREVISIAE AND ANALYSIS OF PROFILIN-DEFICIENT CELLS
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DOI：
10.1083/jcb.110.1.105
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发表时间：
1990-01-01
影响因子：
7.8
通讯作者：
BROWN, SS
中科院分区：
文献类型：
--
作者：
HAARER, BK;LILLIE, SH;BROWN, SS
我们已经从酵母（酿酒酵母）中分离出profilin，并对该蛋白的一部分进行了微测序以确认其身份;微测序的区域与最近从S.啤酒厂（Magdolen，V.，联合Oechsner，G. Muller和W.班德洛1988.摩尔Cell. 8：5108-5115）。酵母profilin在分子量和结合聚脯氨酸、延缓肌动蛋白聚合速率和抑制单体肌动蛋白水解ATP的能力方面类似于来自其他生物体的profilin。使用携带破坏或缺失的profilin基因的菌株，我们已经发现，在适当的条件下，细胞可以在没有可检测到的profilin的情况下存活。这种细胞生长缓慢，对温度敏感，失去酵母细胞的正常椭圆形，通常变成多核，并且通常比野生型细胞大得多。此外，这些细胞表现出细胞壁几丁质的离域沉积，并具有显着改变的肌动蛋白分布。
We have isolated profilin from yeast (Saccharomyces cerevisiae) and have microsequenced a portion of the protein to confirm its identity; the region microsequenced agrees with the predicted amino acid sequence from a profilin gene recently isolated from S. cerevisiae (Magdolen, V., U. Oechsner, G. Muller, and W. Bandlow. 1988. Mol. Cell. Biol. 8:5108-5115). Yeast profilin resembles profilins from other organisms in molecular mass and in the ability to bind to polyproline, retard the rate of actin polymerization, and inhibit hydrolysis of ATP by monomeric actin. Using strains that carry disruptions or deletions of the profilin gene, we have found that, under appropriate conditions, cells can survive without detectable profilin. Such cells grow slowly, are temperature sensitive, loss the normal ellipsoidal shape of yeast cells, often become multinucleate, and generally grow much larger than wild-type cells. In addition, these cells exhibit delocalized deposition of cell wall chitin and have dramatically altered actin distributions.