Noradrenergic modulation of gonadotrophin-inhibitory hormone gene expression in the brain of Japanese quail.
Noradrenergic modulation of gonadotrophin-inhibitory hormone gene expression in the brain of Japanese quail.
复制标题
日本鹌鹑大脑中促性腺激素抑制激素基因表达的去甲肾上腺素调节。
DOI：
10.1111/jne.12503
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发表时间：
2017
期刊：
影响因子：
--
通讯作者：
Tsutsui K.
中科院分区：
文献类型：
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作者：
Tobari Y;Kansaku N;Tsutsui K.
促性腺激素抑制激素（GnIH）是一种下丘脑神经肽，抑制鸟类和哺乳动物促性腺激素的合成和释放。在日本鹌鹑中，GnIH神经元表达去甲肾上腺素能受体并接受去甲肾上腺素能神经支配。去甲肾上腺素（NA）治疗在体外刺激间脑组织块释放GnIH。然而，NA对下丘脑GnIH基因表达的影响尚未确定。本研究采用选择性去甲肾上腺素能神经毒素N-（2-氯乙基）-N-乙基-2-溴苄胺盐酸盐（DSP-4）研究了雄性鹌鹑脑中GnIH基因表达的去甲肾上腺素能调节。我们首先发现DSP-4减少了蓝斑（LoC）中去甲肾上腺素能（多巴胺-β-羟化酶免疫反应）细胞的数量，并特异性降低了雄性鹌鹑下丘脑中的NA浓度。其他单胺，如多巴胺和血清素，不受药物治疗的影响。DSP-4未降低外侧被盖细胞组的去甲肾上腺素能细胞数量，也未降低血浆NA浓度。DSP-4处理后下丘脑NA水平降低不会改变鹌鹑与同种动物相互作用期间大脑中GnIH基因的表达。另一方面，DSP-4处理后，在社会隔离1小时的鹌鹑脑中GnIH基因表达增加。这些结果表明，某些去甲肾上腺素能神经元对鹌鹑下丘脑GnIH基因表达具有抑制作用。
Gonadotrophin‐inhibitory hormone (GnIH) is a hypothalamic neuropeptide that inhibits gonadotrophin synthesis and release in birds and mammals. In Japanese quail, GnIH neurones express the noradrenergic receptor and receive noradrenergic innervation. Treatment with noradrenaline (NA) stimulates GnIH release from diencephalic tissue blocksin vitro. However, the effects of NA on hypothalamic GnIH gene expression have not been determined. We investigated noradrenergic regulation of GnIH gene expression in the brain of male quail using the selective noradrenergic neurotoxinN‐(2‐chloroethyl)‐N‐ethyl‐2‐bromobenzylamine hydrochloride (DSP‐4). We first showed that DSP‐4 reduced the number of noradrenergic (dopamine‐β‐hydroxylase immunoreactive) cells in the locus coeruleus (LoC) and specifically lowered the NA concentration in the hypothalamus of male quail. Other monoamines, such as dopamine and serotonin, were not affected by drug treatment. DSP‐4 did not decrease the numbers of noradrenergic cells of the lateral tegmental cell group, nor the plasma NA concentration. Decreased hypothalamic NA levels after DSP‐4 treatment did not change GnIH gene expression in the brains of quail during their interaction with conspecifics. On the other hand, GnIH gene expression increased in the brains of quail socially isolated for 1 hour after DSP‐4 treatment. These results suggest that some noradrenergic neurones have inhibitory effects on GnIH gene expression of the hypothalamus in solitary quail.