PLD2 is enriched on exosomes and its activity is correlated to the release of exosomes
PLD2 is enriched on exosomes and its activity is correlated to the release of exosomes
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DOI：
10.1016/j.febslet.2004.06.082
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发表时间：
2004-08-13
期刊：
影响因子：
3.5
通讯作者：
Record, M
中科院分区：
文献类型：
--
作者：
Laulagnier, K;Grand, D;Record, M
外泌体是由不同免疫细胞分泌的小囊泡，具有抗肿瘤特性。用离子霉素刺激RBL - 2H3细胞会引发磷脂酶D2（PLD2）从质膜转位到细胞内区室，并释放外泌体。尽管外泌体携带两种PLD异构体，但当在细胞中过表达时，PLD2会富集并特异性地分选到外泌体上。在PLD2过表达后，外泌体上存在的PLD活性明显增加。通过流式细胞术测量，细胞中PLD2的活性与释放的外泌体数量相关。因此，本研究表明外泌体可携带信号传导酶。（C）2004由爱思唯尔B.V.代表欧洲生物化学学会联合会出版。
Exosomes are small vesicles secreted by different immune cells and which display anti-tumoral properties. Stimulation of RBL-2H3 cells with ionomycin triggered phospholipase D2 (PLD2) translocation from plasma membrane to intracellular compartments and the release of exosomes. Although exosomes; carry the two isoforms of PLD, PLD2 was enriched and specifically sorted on exosomes when overexpressed in cells. PLD activity present on exosomes was clearly increased following PLD2 overexpression. PLD2 activity in cells was correlated to the amount of exosome released, as measured by FACS. Therefore, the present work indicates that exosomes can vehicle signaling enzymes. (C) 2004 Published by Elsevier B.V. on behalf of the Federation of European Biochemical Societies.