Effects of lactoferrin on IL-6 production by peritoneal and alveolar cells in cyclophosphamide-treated mice
Effects of lactoferrin on IL-6 production by peritoneal and alveolar cells in cyclophosphamide-treated mice
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DOI：
10.1179/joc.2004.16.2.187
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发表时间：
2004-04-01
影响因子：
1.8
通讯作者：
Kruzel, ML
中科院分区：
文献类型：
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作者：
Artym, J;Zimecki, M;Kruzel, ML
先前的研究表明，口服乳铁蛋白（LF）可恢复环磷酰胺（CP）免疫功能低下小鼠的免疫应答。本研究的目的是确定LF对CP处理小鼠腹腔和肺泡细胞产生白细胞介素6（IL-6）的调节能力。CBA小鼠用单次腹膜内（i. p.）CP剂量（350 mg/kg体重），然后在饮用水（0.5%溶液）中给予LF，持续21天。对照组给予水。从小鼠中分离腹膜和肺泡细胞，并使用生物测定法在24小时细胞培养物中测定自发和脂多糖（LPS）诱导的IL-6的产生。结果表明，在CP处理的小鼠和另外给予LF的小鼠中，IL-6的产生增加。单独给予LF也导致细胞培养物产生IL-6的增加。静脉注射（i. v.）LPS的施用导致CP和CP/LF中IL-6血清水平的显著增加，但在LF处理的小鼠中没有。腹膜腔中细胞类型组成的分析显示CP和CP/LF处理组中肥大细胞和中性粒细胞含量显著增加。我们的研究结果表明，CP和CP/LF处理的小鼠中IL-6的产生增加可能有助于免疫功能低下小鼠免疫系统功能的重建。
Previous studies have shown that oral treatment with lactoferrin (LF) restores the immune response in cyclophosphamide (CP) immunocompromised mice. The aim of the present investigation was to determine the regulatory ability of LF on the production of interleukin 6 (IL-6) in peritoneal and alveolar cells, derived from CP-treated mice. CBA mice were injected with a single, intraperitoneal (i.p.) dose of CP (350 mg/kg body weight) followed by LF administered in drinking water (0.5% solution) for 21 days. The control counterparts were given water. Peritoneal and alveolar cells were isolated from mice and the production of IL-6, both spontaneous and lipopolysaccharide (LPS) induced, was determined in 24h cell cultures using a bioassay. The results showed increased production of IL-6 in both CP-treated mice and in mice given, in addition, LF. The administration of LF alone led also to an increase in IL-6 production by the cell cultures. Intravenous (i.v.) administration of LPS resulted in a significant increase in IL-6 serum levels in CP and CP/LF but not in LF-treated mice. Analysis of cell type composition in the peritoneal cavity revealed a strong increase in mastocyte and neutrophil content in CP and CP/LF-treated groups. Our findings suggest that enhanced IL-6 production in CP and CP/LF-treated mice may contribute to reconstitution of immune system function in immunocompromised mice.