CRISPR/Cas9-Mediated Gene Editing Revolutionizes the Improvement of Horticulture Food Crops.
CRISPR/Cas9-Mediated Gene Editing Revolutionizes the Improvement of Horticulture Food Crops.
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DOI：
10.1021/acs.jafc.1c00104
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发表时间：
2021-03
影响因子：
6.1
通讯作者：
Tian Wang;Chunjiao Zhang;Hongyan Zhang;Hongliang Zhu
中科院分区：
文献类型：
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作者：
Tian Wang;Chunjiao Zhang;Hongyan Zhang;Hongliang Zhu
园艺粮食作物是人类重要的营养来源。随着全球人口的增加，提高园艺粮食作物的产量已成为一项新的挑战，同时也需要丰富其营养成分。基因编辑系统，如锌指核酸酶、转录激活因子样效应物核酸酶和成簇规则间隔短回文重复序列（CRISPR）/CRISPR相关9（Cas9），通过精确修饰靶基因组加速了作物改良。本文综述了各种基因编辑器的发展，并比较了它们的优缺点，特别是新近出现的CRISPR/Cas系统，如碱基编辑和引物编辑。我们还总结了它们在作物性状改良中的实际应用，包括产量，营养品质和其他消费性状。
Horticultural food crops are important sources of nutrients for humans. With the increase of the global population, enhanced horticulture food crop production has become a new challenge, and enriching their nutritional content has also been required. Gene editing systems, such as zinc finger nucleases, transcription activator-like effector nucleases, and clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated 9 (Cas9), have accelerated crop improvement through the modification of targeted genomes precisely. Here, we review the development of various gene editors and compare their advantages and shortcomings, especially the newly emerging CRISPR/Cas systems, such as base editing and prime editing. We also summarize their practical applications in crop trait improvement, including yield, nutritional quality, and other consumer traits.