PNET2 is a component of the plant nuclear lamina and is required for proper genome organization and activity
PNET2 is a component of the plant nuclear lamina and is required for proper genome organization and activity
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DOI：
10.1016/j.devcel.2021.11.002
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发表时间：
2022-01-10
影响因子：
11.8
通讯作者：
Gu, Yangnan
中科院分区：
文献类型：
--
作者：
Tang, Yu;Dong, Qianli;Gu, Yangnan
染色质与核纤层之间的相互作用对于染色质三维结构的建立和基因表达的时空调控具有重要意义。然而，参与这一过程的关键调节剂在植物中知之甚少。在这里，我们报告，拟南芥PNET 2和它的两个同源物是真正的内核膜蛋白和NL的组成部分。PNET 2与植物核骨架物理相互作用，并在核周边接合核小体富集的染色质。所有三个PNET 2的损失导致严重破坏的生长和发育，同时激活非生物和生物胁迫反应，并最终致死拟南芥。pent2三重突变体还显示出剧烈的转录组变化，伴随着HiC分析揭示的全局改变的染色质结构。我们的研究将PNET 2鉴定为NL的内核膜（INM）组分，其与染色质相关，并在植物中协调基因表达和染色质组织中发挥关键作用。
The interaction between chromatin and the nuclear lamina (NL) is intrinsically important to the establishment of three-dimensional chromatin architecture and spatiotemporal regulation of gene expression. However, critical regulators involved in this process are poorly understood in plants. Here, we report that Arabidopsis PNET2 and its two homologs are bona fide inner nuclear membrane proteins and integral components of the NL. PNET2s physically interact with the plant nucleoskeleton and engage nucleosome-enriched chromatin at the nuclear periphery. Loss of all three PNET2s leads to severely disrupted growth and development, concomitant activation of abiotic and biotic stress responses, and ultimate lethality in Arabidopsis. The pent2 triple mutant also displays drastic transcriptome changes accompanied by a globally altered chromatin architecture revealed by HiC analysis. Our study identified PNET2 as an inner nuclear membrane (INM) component of the NL, which associates with chromatin and play a critical role in orchestrating gene expression and chromatin organization in plants.