The chemoreceptor superfamily in the honey bee, Apis mellifera:: Expansion of the odorant, but not gustatory, receptor family
The chemoreceptor superfamily in the honey bee, Apis mellifera:: Expansion of the odorant, but not gustatory, receptor family
复制标题
DOI：
10.1101/gr.5057506
复制
发表时间：
2006-11-01
期刊：
影响因子：
7
通讯作者：
Wanner, Kevin W.
中科院分区：
文献类型：
--
作者：
Robertson, Hugh M.;Wanner, Kevin W.
蜜蜂基因组序列揭示了一个显着的扩展昆虫气味受体（或）家庭相对于剧目的果蝇和冈比亚按蚊，分别有62个和79个或。在蜜蜂中总共注释了170个Or基因，其中7个是假基因。这些基因组成了昆虫Or家族树中的5个蜜蜂特异性亚家族，其中一个已经扩展到总共157个基因，编码具有15%-99%氨基酸同一性的蛋白质。大多数Or基因都在串联阵列中，包括一个有60个基因的阵列。这种蜜蜂特有的扩大或剧目大概是其显着的嗅觉能力的基础，包括感知几种信息素混合物，亲属识别信号，和不同的花香。西方蜜蜂的数量大约等于蜜蜂触角叶中肾小球的数量（160-170），与一般的一个受体/一个神经元/一个肾小球的关系一致。蜜蜂的基因组只编码10种味觉受体（Grs），而D。melanogaster和A.冈比亚的剧目分别为68和76 Grs。缺乏Gr基因家族扩展主要是这种差异的原因。培育蜂巢环境和与植物的互利关系可以解释Gr家族扩张的缺乏。Or家族是蜜蜂基因组中基因家族扩张的最引人注目的例子，并且表征其种姓和性别特异性基因表达可能为它们在检测信息素、亲属和花香中的特定作用提供线索。
The honey bee genome sequence reveals a remarkable expansion of the insect odorant receptor ( Or) family relative to the repertoires of the flies Drosophila melanogaster and Anopheles gambiae, which have 62 and 79 Ors respectively. A total of 170 Or genes were annotated in the bee, of which seven are pseudogenes. These constitute five bee-specific subfamilies in an insect Or family tree, one of which has expanded to a total of 157 genes encoding proteins with 15%-99% amino acid identity. Most of the Or genes are in tandem arrays, including one with 60 genes. This bee-specific expansion of the Or repertoire presumably underlies their remarkable olfactory abilities, including perception of several pheromone blends, kin recognition signals, and diverse floral odors. The number of Apis mellifera Ors is approximately equal to the number of glomeruli in the bee antennal lobe (160-170), consistent with a general one-receptor/one-neuron/one-glomerulus relationship. The bee genome encodes just 10 gustatory receptors (Grs) compared with the D. melanogaster and A. gambiae repertoires of 68 and 76 Grs, respectively. A lack of Gr gene family expansion primarily accounts for this difference. A nurturing hive environment and a mutualistic relationship with plants may explain the lack of Gr family expansion. The Or family is the most dramatic example of gene family expansion in the bee genome, and characterizing their caste- and sex-specific gene expression may provide clues to their specific roles in detection of pheromone, kin, and floral odors.