Response of Selenium and Selenogenome in Immune Tissues to LPS-Induced Inflammatory Reactions in Pigs
Response of Selenium and Selenogenome in Immune Tissues to LPS-Induced Inflammatory Reactions in Pigs
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猪免疫组织中硒和硒基因组对脂多糖诱导的炎症反应的反应
DOI：
10.1007/s12011-016-0863-4
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发表时间：
2017-05-01
影响因子：
3.9
通讯作者：
Liu, Yu-Lan
中科院分区：
文献类型：
--
作者：
Sun, Lv-Hui;Pi, Ding-An;Liu, Yu-Lan
在炎症性疾病中，必需微量元素硒（Se）的循环浓度显著降低。虽然硒主要通过25种硒蛋白发挥生理作用，但硒基因组在免疫组织炎症反应中的反应仍不清楚。本研究的目的是确定硒潴留和硒基因组表达在免疫组织中的脂多糖（LPS）诱导的炎症反应在猪。将12头雄性猪随机分为两组，分别注射LPS和生理盐水。注射后4 h，采集血样并对猪实施安乐死。LPS组血清和脾脏硒含量分别比生理盐水组低36.8%和16.6%（P< 0.05）。注射LPS后，猪血清、胸腺和淋巴结中GPX活性分别下降23.4%、26.6%和30.4%（P< 0.05）。LPS刺激后，肝脏、脾脏、胸腺和淋巴结中分别有14、16、10和6个硒蛋白基因的mRNA表达发生显著变化（P< 0.05）。沿着10个已报道的硒蛋白基因，本研究首次阐明了Txnrd2、Txnrd3、Sep15、Selh、Seli、Seln、Selo、Selt、Selx和Sephs2等基因在免疫组织炎症反应中的作用。总之，LPS诱导的炎症反应损害了硒代谢，并与免疫组织中硒基因组表达失调有关。
Circulating concentration of the essential trace element selenium (Se) was significantly lower in inflammatory disorders. Although Se plays physiological roles mainly through the function of 25 selenoproteins, the response of the selenogenome in immune tissues during inflammatory reactions remains unclear. The objective of this study was to determine the Se retention and selenogenome expression in immune tissues during the lipopolysaccharide (LPS)-induced inflammatory response in porcine. A total of 12 male pigs were randomly divided into two groups and injected with LPS or saline. After 4 h postinjection, blood samples were collected and pigs were euthanized. Pigs challenged with LPS had 36.8 and 16.6 % lower (P< 0.05) Se concentrations in the serum and spleen, respectively, than those injected with saline. Moreover, the activities of GPX decreased (P< 0.05) by 23.4, 26.6, and 30.4 % in the serum, thymus, and lymph node, respectively, in the pigs injected with LPS. Furthermore, the LPS challenge altered (P< 0.05) the mRNA expression of 14, 16, 10, and 6 selenoprotein genes in the liver, spleen, thymus, and lymph node, respectively. Along with 10 previously reported selenoprotein genes, the response ofTxnrd2,Txnrd3,Sep15,Selh,Seli,Seln,Selo,Selt,Selx, andSephs2to inflammatory reaction in immune tissues were newly illustrated in this study. In conclusion, the LPS-induced inflammatory response impaired Se metabolism and was associated with dysregulation of the selenogenome expression in immune tissues.