The combined effect of brain-derived neurotrophic factor and a free radical scavenger in experimental glaucoma.
The combined effect of brain-derived neurotrophic factor and a free radical scavenger in experimental glaucoma.
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发表时间：
2000-09
影响因子：
4.4
通讯作者：
Mei-Lan Ko;Mei-Lan Ko;Dan-ning Hu;Dan-ning Hu;R. Ritch;R. Ritch;Sansar C. Sharma
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文献类型：
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作者：
Mei-Lan Ko;Mei-Lan Ko;Dan-ning Hu;Dan-ning Hu;R. Ritch;R. Ritch;Sansar C. Sharma
未标明用途。脑源性神经营养因子（BDNF）对高眼压大鼠视网膜神经节细胞（RGC）的存活影响有限。本实验观察了BDNF与非特异性自由基清除剂N-叔丁基-（2-磺基苯）-硝酮（S-PBN）联合应用对高血压大鼠视网膜节细胞（RGCs）的影响。方法成年Wistar大鼠随机分为5组：BDNF（0. 5 μ g）+ S-PBN组、BDNF（1. 5 μ g）+ S-PBN组、BDNF（0. 5 μ g）+ S-PBN组。0微克）+ S-PBN; BDNF（1.0微克）; S-PBN;和磷酸盐缓冲盐水。右眼作为正常对照（n = 10）。用5% Fluoro Gold标记RGC;注射到上级丘中。顶盖内注射后3天，烧灼左眼的巩膜外静脉。在IOP升高后第5、13、21和29天进行BDNF玻璃体内注射。从烧灼后30分钟开始，每12小时腹膜内注射S-PBN（100 mg/kg体重）。结果在中度高血压眼中，单独使用BDNF处理的RGCs的存活和单独使用S-PBN的全身给药不能显著挽救RGCs。然而，BDNF和S-PBN的组合将RGCs的存活率提高到90.1%。结论营养因子和抗氧化剂对高眼压眼RGCs的死亡具有协同作用。对不同联合治疗疗法的进一步研究可能提供在IOP升高的眼睛中挽救RGC免于死亡的途径。
UNLABELLED PURPOSE. Brain-derived neurotrophic factor (BDNF) had a limited effect on the survival of retinal ganglion cells (RGCs) in rats' eyes with elevated intraocular pressure (IOP). The combined treatment of BDNF and a nonspecific free radical scavenger N-tert-butyl-(2-sulfophenyal)-nitrone (S-PBN) was investigated on the RGCs in hypertensive eyes of rats. METHODS Adult Wistar rats were separated into five groups: BDNF (0.5 microg) + S-PBN; BDNF (1. 0 microg) + S-PBN; BDNF (1.0 microg); S-PBN; and phosphate-buffered saline. Right eyes served as normal controls (n = 10). RGCs were labeled with 5% Fluoro Gold; injected into the superior colliculus. Three days after intratectal injection, the episcleral veins of the left eyes were cauterized. Intravitreal injection of BDNF was performed on days 5, 13, 21, and 29 after IOP elevation. S-PBN was injected intraperitoneally (100 mg/kg body wt) every 12 hours starting 30 minutes after cauterization. RESULTS The survival of RGCs using BDNF treatment alone in moderately hypertensive eyes and systemic administration of S-PBN alone did not significantly rescue the RGCs. However, the combination of BDNF and S-PBN increased the survival of RGCs to 90.1%. CONCLUSIONS Trophic factors and antioxidants have synergistic effects on rescuing RGCs from death in eyes with elevated IOP. Further studies of different combined treatment therapies may provide avenues to save RGCs from death in eyes with elevated IOP.