Pseudo-nitzschia species, a possible causative organism of domoic acid in Spondylus versicolor collected from Nha Phu Bay, Khanh Hoa Province, Vietnam
Pseudo-nitzschia species, a possible causative organism of domoic acid in Spondylus versicolor collected from Nha Phu Bay, Khanh Hoa Province, Vietnam
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发表时间：
2012-03
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通讯作者：
Viet Ha Dao;T. Omura;Y. Takata;X. Pham;Y. Fukuyo;M. Kodama
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作者：
Viet Ha Dao;T. Omura;Y. Takata;X. Pham;Y. Fukuyo;M. Kodama
最近，有报道称，在从越南等多个热带地区采集的海蛞蝓属双壳贝类中检测到了大量的软骨藻酸（一种导致失忆性贝类中毒（ASP）的毒素），而在其他双壳贝类中没有检测到显着的软骨藻酸。这些事实表明，软骨藻酸的病原浮游生物种类广泛存在于热带沃茨，脊椎虫属（Spondylus spp.）比其他双壳类更有效地积累软骨藻酸。在对S.在越南的Nha Phu湾，S. vesicolor和浮游生物网样中，软骨藻酸的水平。当浮游生物网样品中检测到软骨藻酸时，发现变色龙增加。在光镜下观察浮游生物样品，其大小小于有毒的假菱形藻如多系假菱形藻。当浮游生物网样品通过具有不同孔径的筛和膜过滤器的连续过滤而分级时，大部分软骨藻酸在具有最小尺寸（0.6-10 mm）的颗粒级分中回收。从该级分中的细胞建立的单细胞培养物的一些菌株显示软骨藻酸的产生，尽管生产率低。所有的软骨藻酸产生菌似乎是同一种。这些结果表明，伪菱形藻种也是导致软骨藻酸在热带双壳类。
Recently, it was reported that a significant level of domoic acid, a causative toxin of amnesic shellfish poisoning (ASP), was detected in bivalves belonging to the genus Spondylus collected from various tropical areas, including Vietnam, whereas no significant domoic acid was detected in other bivalve species. These facts suggest that the causative plankton species for domoic acid occur widely in tropical waters and that Spondylus spp. accumulate domoic acid more effectively than other bivalve species. In the monitoring on seasonal change of domoic acid levels in S. vesicolor and plankton net samples in Nha Phu Bay, Vietnam, domoic acid level of S. versicolor was found to increase when domoic acid was detected in the plankton net samples. Under light microscopic observation of the plankton samples, cells of Pseudo-nitzschia spp., the size of which was smaller than toxic Pseudo- nitzschia such as P. multiseries, were observed. When the plankton net samples were fractionated by successive filtration through the sieves and membrane filters with different pore sizes, most of the domoic acid was recovered in the particle fraction with smallest size (0.6-10 mm). Some strains of unicellular cultures established from the cells in this fraction showed the production of domoic acid, though the productivity was low. All the domoic acid-producing strains seemed to be the same species. These results indicate that Pseudo-nitzschia species is also causative for domoic acid in tropical bivalves.