Two tonoplast proton pumps function in Arabidopsis embryo development
Two tonoplast proton pumps function in Arabidopsis embryo development
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两个液泡膜质子泵在拟南芥胚胎发育中发挥作用
DOI：
10.1111/nph.16231
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发表时间：
2019-11-14
期刊：
影响因子：
9.4
通讯作者：
Lin, Wen-Hui
中科院分区：
文献类型：
--
作者：
Jiang, Yu-Tong;Tang, Ren-Jie;Lin, Wen-Hui
两种类型的张力质体质子泵，H+-焦磷酸酶（V-PPase）和H+- atp酶（V-ATPase），建立质子梯度，为张力质体的分子交通提供动力，从而促进肿胀调节和营养稳态。然而，质子泵如何调节发育仍不清楚。在本研究中，我们研究了两种质子泵在拟南芥胚发育和模式形成中的作用。破坏V-PPase或V-ATPase对植物胚胎发育均无明显影响，但敲除这两种酶会导致胚胎早期形态形成严重缺陷。野生型受精卵的第一次分裂是不对称的，而突变体发生了几乎对称的分裂，随后在胚胎发育的各个阶段都出现了异常的模式形成。胚胎缺陷伴随着液泡形态和分布的巨大差异，以及PIN1的定位受到干扰。突变体子叶和根的发育以及突变体幼苗对生长素的反应支持了缺乏张力质体质子泵的突变体在生长素运输和分配方面存在缺陷的假设。综上所述，我们提出在拟南芥胚胎和幼苗中液泡形态和PIN1定位需要两个细胞体质子泵，从而控制液泡和生长素相关的发育过程。
Two types of tonoplast proton pumps, H+-pyrophosphatase (V-PPase) and the H+-ATPase (V-ATPase), establish the proton gradient that powers molecular traffic across the tonoplast thereby facilitating turgor regulation and nutrient homeostasis. However, how proton pumps regulate development remains unclear. In this study, we investigated the function of two types of proton pumps in Arabidopsis embryo development and pattern formation. While disruption of either V-PPase or V-ATPase had no obvious effect on plant embryo development, knocking out both resulted in severe defects in embryo pattern formation from the early stage. While the first division in wild-type zygote was asymmetrical, a nearly symmetrical division occurred in the mutant, followed by abnormal pattern formation at all stages of embryo development. The embryonic defects were accompanied by dramatic differences in vacuole morphology and distribution, as well as disturbed localisation of PIN1. The development of mutant cotyledons and root, and the auxin response of mutant seedlings supported the hypothesis that mutants lacking tonoplast proton pumps were defective in auxin transport and distribution. Taking together, we proposed that two tonoplast proton pumps are required for vacuole morphology and PIN1 localisation, thereby controlling vacuole and auxin-related developmental processes in Arabidopsis embryos and seedlings.