Is protein folding hierarchic? II. Folding intermediates and transition states
Is protein folding hierarchic? II. Folding intermediates and transition states
复制标题
DOI：
10.1016/s0968-0004(98)01345-0
复制
发表时间：
1999-02-01
影响因子：
13.8
通讯作者：
Rose, GD
中科院分区：
文献类型：
--
作者：
Baldwin, RL;Rose, GD
一些小蛋白质的折叠反应显示了等级过程的明确证据，而另一些由于缺乏可检测的中间物，则没有。来自折叠中间体和过渡态的证据表明，折叠始于局部，原生二级结构的形成先于三级相互作用的形成，而不是相反。文献中一些值得注意的例子被解释为相反。对于这些示例，我们通过使用关闭非局部相互作用的LINUS程序模拟了折叠开始时形成的局部结构。我们的结果支持蛋白质折叠的层次模型。
The folding reactions of some small proteins show clear evidence of a hierarchic process, whereas others, lacking detectable intermediates, do not. Evidence from folding intermediates and transition states suggests that folding begins locally, and that the formation of native secondary structure precedes the formation of tertiary interactions, not the reverse. Some notable examples in the literature have been interpreted to the contrary. For these examples, we have simulated the local structures that form when folding begins by using the LINUS program with nonlocal interactions turned off. Our results support a hierarchic model of protein folding.