Blood agar and Mycobacterium tuberculosis:: the end of a dogma
Blood agar and Mycobacterium tuberculosis:: the end of a dogma
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DOI：
10.1128/jcm.41.4.1710-1711.2003
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发表时间：
2003-04-01
影响因子：
9.4
通讯作者：
Raoult, D
中科院分区：
文献类型：
--
作者：
Drancourt, M;Carrieri, P;Raoult, D
结核分枝杆菌的偶然血琼脂复苏使我们进一步研究了这种常规的结核分枝杆菌分离和培养方法。将15个呼吸道和8个淋巴结Ziehl-Neelsen阳性标本平行接种到含有蛋基培养基和5%绵羊血琼脂的试管中。在此基础上的集落形成时间比以鸡蛋为基础的培养基快，但差异不显著(P=0.11，方差分析[ANOVA]检验)。进一步的实验比较了38株呼吸道和淋巴结核分支杆菌分离株在这两种培养基上传代培养时的生长情况。培养6天后，38株菌中有21株在血琼脂上生长，且血琼脂上的平均菌落数显著高于鸡蛋培养基上的平均菌落数(P<；0.001，方差分析)。这些结果表明，结核分枝杆菌在血琼脂上1~2周内即可生长，说明该基本培养基除适用于其他培养基外，还适用于结核病的实验室诊断。实验室经常使用长时间的血板孵育，例如，用于巴尔通体的恢复，应该考虑遇到这种高度传染性病原体的潜在安全影响。
Incidental blood agar-based recovery of Mycobacterium tuberculosis led us to further investigate this routine medium for primary isolation and culture of M. tuberculosis. Fifteen respiratory tract and eight lymph node Ziehl-Neelsen-positive specimens were inoculated in parallel into tubes containing egg-based medium and 5% sheep blood agar. Colonies appeared sooner on this medium than on the egg-based medium, but this difference was not significant (P = 0.11, analysis of variance [ANOVA] test). Further experiments compared the growth of 38 respiratory and lymph node M. tuberculosis isolates when subcultured on the two media. After 6 days of incubation, 21 of 38 isolates had grown on blood agar, and the mean number of colonies was significantly greater on blood agar than on the egg-based medium (P < 0 0.001, ANOVA test). These results demonstrate that M. tuberculosis grows easily on blood agar within 1 to 2 weeks, indicating that this basic medium is suitable for laboratory diagnosis of tuberculosis in addition to other media. Laboratories that routinely use prolonged incubations of blood plates, for example, for the recovery of Bartonella species, should consider the potential safety implications of encountering this highly infectious pathogen.