Heterotypic transcriptional condensates formed by prion-like paralogous proteins canalize flowering transition in tomato.
Heterotypic transcriptional condensates formed by prion-like paralogous proteins canalize flowering transition in tomato.
复制标题
由类朊病毒旁系同源蛋白形成的异型转录缩合物促进番茄开花转变
DOI：
10.1186/s13059-022-02646-6
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发表时间：
2022-03-14
期刊：
影响因子：
12.3
通讯作者：
Xu C
中科院分区：
文献类型：
--
作者：
Huang X;Xiao N;Zou Y;Xie Y;Tang L;Zhang Y;Yu Y;Li Y;Xu C
背景在基因组进化过程中，由于基因复制而产生的同源基因使遗传冗余和表型稳健性成为可能。类似转录调控因子的编码或调控序列的变异使它们的功能和关系多样化，这为发育提供了抵御遗传或环境干扰的稳健性。植物茎干细胞开花和繁殖成功的命运转换需要强大的转录调控。结果我们探索了不同转录丰度的Alog家族转录因子在茎分生组织中的遗传关系和蛋白质行为。突变谱涵盖了5个Alog亲缘关系的单一和更高级别的突变组合，并产生了开花过渡缺陷的连续体，表现出逐渐增强的早熟开花，以及花序简化，从野生型到逐渐减少的花，直到具有不育花器官的单花。因此，这些Paralog扮演着不平等的角色，并共同行动起来，实现强大的遗传管道化。这五种蛋白质都含有类Pron的固有无序区(IDR)，并经历了相分离。基因复制后累积的突变导致Alog同源基因之间的IDR变异，导致不同的相分离和转录调节能力。值得注意的是，它们保留了祖先组装成异型冷凝物的能力，防止了花身份基因ANANTHA的早熟激活。结论我们的研究揭示了一种新的遗传教化机制，这种机制是由平行蛋白相互作用和相分离形成的异型转录冷凝物实现的，揭示了基因复制导致IDR变异和干细胞命运的强大转录控制之间的分子联系。
BackgroundParalogs that arise from gene duplications during genome evolution enable genetic redundancy and phenotypic robustness. Variation in the coding or regulatory sequence of paralogous transcriptional regulators diversifies their functions and relationships, which provides developmental robustness against genetic or environmental perturbation. The fate transition of plant shoot stem cells for flowering and reproductive success requires a robust transcriptional control. However, how paralogs function and interact to achieve such robustness is unknown.ResultsHere, we explore the genetic relationship and protein behavior of ALOG family transcriptional factors with diverse transcriptional abundance in shoot meristems. A mutant spectrum covers single and higher-order mutant combinations of five ALOG paralogs and creates a continuum of flowering transition defects, showing gradually enhanced precocious flowering, along with inflorescence simplification from wild-type-like to progressively fewer flowers until solitary flower with sterile floral organs. Therefore, these paralogs play unequal roles and act together to achieve a robust genetic canalization. All five proteins contain prion-like intrinsically disordered regions (IDRs) and undergo phase separation. Accumulated mutations following gene duplications lead to IDR variations among ALOG paralogs, resulting in divergent phase separation and transcriptional regulation capabilities. Remarkably, they retain the ancestral abilities to assemble into a heterotypic condensate that prevents precocious activation of the floral identity geneANANTHA.ConclusionsOur study reveals a novel genetic canalization mechanism enabled by heterotypic transcriptional condensates formed by paralogous protein interactions and phase separation, uncovering the molecular link between gene duplication caused IDR variation and robust transcriptional control of stem cell fate transition.
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影响因子：
4.3
作者：
Hepworth, SR;Klenz, JE;Haughn, GW
通讯作者：
Haughn, GW
影响因子：
56.9
作者：
Franzmann, Titus M.;Jahnel, Marcus;Alberti, Simon
通讯作者：
Alberti, Simon
矮牵牛 ALOG 基因家族的全基因组鉴定和表征
影响因子：
5.3
作者：
Chen, Feng;Zhou, Qin;Liu, Guofeng
通讯作者：
Liu, Guofeng
影响因子：
3.5
作者：
Nan W;Shi S;Jeewani DC;Quan L;Shi X;Wang Z
通讯作者：
Wang Z
影响因子：
64.8
作者：
Fang, Xiaofeng;Wang, Liang;Dean, Caroline
通讯作者：
Dean, Caroline