The major capsid protein of the lipid-containing bacteriophage PR4 is the precursor of two other capsid proteins.
The major capsid protein of the lipid-containing bacteriophage PR4 is the precursor of two other capsid proteins.
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含脂噬菌体 PR4 的主要衣壳蛋白是其他两种衣壳蛋白的前体。
DOI：
10.1006/viro.1994.1003
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发表时间：
1994
期刊：
影响因子：
3.7
通讯作者：
CronanJr,JE
中科院分区：
文献类型：
--
作者：
Myung,H;VandenBoom,T;CronanJr,JE
我们报道了噬菌体PR4的衣壳蛋白P16和P18是由主要衣壳蛋白P2的一部分翻译后加工而成的。纯化的P2多克隆抗体可与P16、P18反应，也可与P2反应。一株单抗与P2和P18均有反应。P2和P18的N-末端氨基酸序列完全匹配，表明P18来源于P2的N-末端片段。对各种无义和错义P2突变体编码的蛋白质的分析证实了这些数据。PR4基因组的3129-bpMnlI-C片段编码P2。获得了该片段的核苷酸序列，并发现编码P2的单个连续ORF，从而排除了P16和P18生产中的内含子和转录加工。该DNA片段包含8个大小为200 bp的开放阅读框，并通过标记拯救分析定位了编码蛋白P6、P6A和P2的基因。我们还报道了一类新的P2错义突变体的分离和鉴定。
We report that capsid proteins P16 and P18 of bacteriophage PR4 are synthesized by post-translational processing of a portion of the major capsid protein, P2. A polyclonal antibody raised against purified P2 reacted with P16 and P18 as well as with P2. A monoclonal antibody reacted with both P2 and P18. The amino acid sequences of the N-termini of P2 and P18 exactly matched, indicating that P18 is derived from the N-terminal segment of P2. These data were confirmed by the analysis of the proteins encoded by various nonsense and missense P2 mutants. The 3129-bpMnlI-C fragment of the PR4 genome was shown to encode P2. The nucleotide sequence of this fragment was obtained and a single continuous ORF was found to encode P2, thus excluding introns and transcript processing in the production of P16 and P18. The DNA segment contained eight ORFs sized >200 bp and the genes encoding proteins P6 and P6A as well as P2 were mapped by marker rescue analysis. We also report the isolation and characterization of a new class of P2 missense mutants.