Over-expression of fibroblast growth factor receptor 3 in human hepatocellular carcinoma
Over-expression of fibroblast growth factor receptor 3 in human hepatocellular carcinoma
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DOI：
10.3748/wjg.v11.i34.5266
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发表时间：
2005-09-14
影响因子：
4.3
通讯作者：
Yen, Yun
中科院分区：
文献类型：
--
作者：
Qiu, Wei-Hua;Zhou, Bing-Sen;Yen, Yun
目的：目的：探讨信号转导和细胞增殖相关基因成纤维细胞生长因子受体3（fibroblast growth factor receptor 3，FGFR 3）在肝细胞癌（hepatocellular carcinoma，HCC）中的过度表达。结果：北方杂交结果显示肝癌组织中FGFR 3明显过表达，这与DNA微阵列的结果一致。实时荧光定量PCR显示，肝癌组织中FGFR 3 mRNA与甘油醛-3-磷酸脱氢酶（GADPH）mRNA的平均比值为0.250，而非肿瘤性肝组织中的比值为0.014。对43例HCC的统计学分析显示，HCC的评分高于匹配的非肿瘤性肝组织。FGFR 3的过度表达与肝癌的分化程度和核分级密切相关。结论：FGFR 3的过度表达可能在肝癌的发生发展中起重要作用。FGFR 3有可能成为肝癌诊断的理想分子标志物和潜在的治疗靶点。(C)2005年WJG出版社和Elsevier Inc. All rights reserved.
AIM: To describe the significant over-expression of fibroblast growth factor receptor 3 (FGFR3), which is a signal transduction and cell proliferation related gene in hepatocellular carcinoma (HCC).METHODS: Following DNA microarray, Northern blot and quantitative real-time PCR were employed to confirm FGFR3 expression difference in HCC tissues and surrounding non-neoplastic liver tissue. FGFR3 expression levels were further determined by immunohistochemical study in 43 cases of HCC.RESULTS: Northern blot results showed the significant over-expression of FGFR3 in HCC tissues, which was consistent with that from DNA microarray. Quantitative real-time PCR demonstrated that the mean ratio of FGFR3 mRNA to glyceraldehyde-3-phosphate dehydrogenase (GADPH) mRNA in HCC tissue was 0.250, whereas the ratio in non-neoplastic liver tissue was 0.014. Statistical analyses of 43 cases of HCC revealed that HCC scored higher than the matched non-neoplastic liver tissues. Examination of clinicopathological features revealed a strong correlation of over-expression of FGFR3 with poor tumor differentiation and high nuclear grade.CONCLUSION: Over-expression of FGFR3 may play an important role in liver carcinogenesis. FGFR3 may be an ideal candidate as a molecular marker in the diagnosis of HCC and a potential therapeutic target. (C) 2005 The WJG Press and Elsevier Inc. All rights reserved.