Ultrastructure of hair follicle afferent fibre terminations in the spinal cord of the cat
Ultrastructure of hair follicle afferent fibre terminations in the spinal cord of the cat
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猫脊髓毛囊传入纤维末端的超微结构
DOI：
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发表时间：
1982
期刊：
影响因子：
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通讯作者：
A. G. Brown
中科院分区：
文献类型：
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作者：
D. Maxwell;B. A. Bannatyne;R. Fyffe;A. G. Brown
在麻醉猫的急性电生理实验中，用辣根过氧化物酶（HRP）注射单个毛囊传入纤维。HRP从腰骶髓轴突内微电极注入。注射后1至6小时，对动物进行灌注，并制备组织用于光学和电子显微镜检查（EM）。在光学显微镜下厚切片鉴定含有HRP反应产物的轴突侧支分支，然后在超薄切片机上切割相同的组织用于EM研究。侧支的终末支在形成突触终扣前仍保留髓鞘，直至直径为0.45 ~ 1.0 μm。突触终扣（直径1.0-4.0 μm）多为过渡型，与树突接触。接触是不对称的（I型），包含直径为35-60 nm的圆形、透明突触囊泡。终末轴突的无髓鞘部分和突触终末都有轴-轴接触。这些轴突轴突终扣含有清晰的（无颗粒）囊泡不规则的轮廓。
SummaryIn acute electrophysiological experiments on anaesthetized cats, single identified hair follicle afferent fibres were injected with horseradish peroxidase (HRP). The HRP was injected from an intra-axonal microelectrode in the lumbosacral spinal cord. One to six hours after injection the animals were perfused and the tissue prepared for light and electron microscopy (EM). Axon collateral arborizations containing HRP reaction product were identified in thick sections under the light microscope and the same tissue then cut on the ultramicrotome for EM study. The terminal branches of the collaterals kept their myelin sheaths until they were 0.45–l.0 μm in diameter, just before they formed synaptic boutons. Synaptic boutons (1.0–4.0 μm in diameter) were usually of theen passant variety and made contact with dendrites. The contacts were asymmetrical (Type I) and contained round, clear synaptic vesicles of 35–60 nm diameter. Both the non-myelinated portion of the terminal axon and the synaptic boutons received axo-axonic contacts. These axo-axonic boutons contained clear (agranular) vesicles irregular in profile.