DIVERSITY OF THE CADHERIN FAMILY - EVIDENCE FOR 8 NEW CADHERINS IN NERVOUS-TISSUE
DIVERSITY OF THE CADHERIN FAMILY - EVIDENCE FOR 8 NEW CADHERINS IN NERVOUS-TISSUE
复制标题
DOI：
10.1091/mbc.2.4.261
复制
发表时间：
1991-04-01
期刊：
影响因子：
--
通讯作者：
TANIHARA, H
中科院分区：
文献类型：
--
作者：
SUZUKI, S;SANO, K;TANIHARA, H
为了研究钙粘附素家族的多样性，我们利用聚合酶链式反应从脑和视网膜的cDNAs中分离出cDNA。获得的产物包括三个已知钙粘蛋白中的两个的cDNA以及八个不同的cDNA，其中推导的氨基酸序列与已知的钙粘蛋白序列具有显著的相似性。在这八个新分子中，有六个是从人的cdna文库中分离到的较大的cdna克隆。推导的氨基酸序列表明，这些分子的整体结构与已知的钙粘蛋白非常相似，表明这些分子是钙粘蛋白家族的新成员。我们初步将这些钙粘素命名为钙粘素-4至-11。这些新分子，除了钙粘素-4，表现出不同于以前克隆的钙粘附素的特征；它们可能属于一个新的钙粘附素亚家族。Northern印迹分析表明，这些钙粘附素大部分主要在脑内表达，但也有一些在其他组织中表达。这些发现表明，钙粘附素家族的黏附分子比之前认为的要大得多，并表明新的钙粘附素可能在中枢神经系统内的细胞与细胞之间的相互作用中发挥重要作用。
To examine the diversity of the cadherin family, we isolated cDNAs from brain and retina cDNA preparations with the aid of polymerase chain reaction. The products obtained included cDNAs for two of three known cadherins as well as eight distinct cDNAs, of which deduced amino acid sequences show significant similarity with the known cadherin sequences. Larger cDNA clones were isolated from human cDNA libraries for six of the eight new molecules. The deduced amino acid sequences show that the overall structure of these molecules is very similar to that of the known cadherins, indicating that these molecules are new members of the cadherin family. We have tentatively designated these cadherins as cadherin-4 through -11. The new molecules, with the exception of cadherin-4, exhibit features that distinguish them as a group from previously cloned cadherins; they may belong to a new subfamily of cadherins. Northern blot analysis showed that most of these cadherins are expressed mainly in brain, although some are expressed in other tissues as well. These findings show that the cadherin family of adhesion molecules is much larger than previously thought, and suggest that the new cadherins may play an important role in cell-cell interactions within the central nervous system.