Analysis of ABCC6 (MRP6) in normal human tissues
Analysis of ABCC6 (MRP6) in normal human tissues
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DOI：
10.1007/s00418-004-0744-3
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发表时间：
2005-06-01
影响因子：
2.3
通讯作者：
Boyd, CD
中科院分区：
文献类型：
--
作者：
Beck, K;Hayashi, K;Boyd, CD
为了确定ABC转运蛋白ABCC 6在正常人体组织中的组织分布，我们使用多克隆抗体HB-6通过原位杂交和免疫组织化学分析了组织阵列中ABCC 6 mRNA和ABCC 6蛋白的存在。我们检测了ABCC 6 mRNA和蛋白质的各种上皮细胞的外分泌和内分泌组织，如腺泡细胞在胰腺，肠粘膜细胞和滤泡上皮细胞的甲状腺。我们在胃中获得了非常强的肠内分泌G细胞免疫染色。此外，ABCC 6 mRNA和蛋白质存在于脑的大多数神经元、肺的肺泡巨噬细胞和淋巴结的淋巴细胞中。使用单克隆抗体M6 II-31的免疫组织化学证实了ABCC 6的广泛组织分布。ABCC 6的生理底物尚不清楚，但我们认为ABCC 6在不同组织中具有多种功能。ABCC 6在G细胞中的强免疫染色表明其在这些内分泌细胞中起重要作用。
To determine the tissue distribution of the ABC transporter ABCC6 in normal human tissues, we analyzed tissue arrays for the presence of ABCC6 mRNA by in situ hybridization and ABCC6 protein by immunohistochemistry using the polyclonal antibody HB-6. We detected ABCC6 mRNA and protein in various epithelial cells of exocrine and endocrine tissues, such as acinar cells in the pancreas, mucosal cells of the intestine and follicular epithelial cells of the thyroid. We obtained a very strong immunostaining for enteroendocrine G cells in the stomach. In addition, ABCC6 mRNA and protein were present in most neurons of the brain, in alveolar macrophages in the lungs and lymphocytes in the lymph node. Immunohistochemisty using the monoclonal antibody M6II-31 confirmed the widespread tissue distribution of ABCC6. The physiological substrate(s) of ABCC6 are yet unknown, but we suggest that ABCC6 fulfills multiple functions in different tissues. The strong immunostaining for ABCC6 in G cells suggests that it plays an important role in these endocrine cells.