EXPRESSION OF EPSTEIN-BARR-VIRUS-RELATED NUCLEAR ANTIGENS AND B-CELL MARKERS IN LYMPHOMAS OF SIV-IMMUNOSUPPRESSED MONKEYS
EXPRESSION OF EPSTEIN-BARR-VIRUS-RELATED NUCLEAR ANTIGENS AND B-CELL MARKERS IN LYMPHOMAS OF SIV-IMMUNOSUPPRESSED MONKEYS
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DOI：
10.1002/ijc.2910550416
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发表时间：
1993-10-21
影响因子：
6.4
通讯作者：
ERNBERG, I
中科院分区：
文献类型：
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作者：
LI, SL;FEICHTINGER, H;ERNBERG, I
感染猿猴免疫缺陷病毒 (SIV) 的食蟹猴大约有三分之一的病例会患上 B 细胞淋巴瘤。我们现在研究了 13 种携带 SIVsm 相关猴淋巴瘤的食蟹猴 Epstein-Barr 病毒 (cyno-EBV) 核抗原的表达，并从其中 3 种肿瘤中建立了细胞系。使用针对人 EB 病毒 EBNA1-6 和膜蛋白 LMP1 的多特异性和单特异性试剂探测细胞裂解物的免疫印迹。 EBNA2 交叉反应蛋白在 8 个淋巴瘤组织 (8/13) 和 3 个源自肿瘤的细胞系中得到证实。所有肿瘤均表达分子量为 55 kDa 的多肽，该多肽与一些 EBNAI 抗体发生交叉反应。正常猴组织的吸收实验表明，这种多肽对携带 cyno-EBV 的淋巴瘤细胞具有特异性。无法检测到 EBNA3-6 和 LMP1 的等效物。免疫表型特征表明，猴淋巴瘤与人类 HIV 相关 B 细胞淋巴瘤相似。实验性感染 SIVsm 的食蟹猴中的恶性 B 细胞淋巴瘤可以作为免疫缺陷状态下 EBV 相关淋巴瘤发生的模型。 (C) 1993 Wiley-Liss, Inc.
Simian-immunodeficiency-virus(SIV)-infected cynomolgus monkeys develop B-cell lymphomas in approximately one third of the cases. We have now studied the expression of cynomolgus-Epstein-Barr-virus(cyno-EBV) nuclear antigens in 13 cyno-EBV-carrying SIVsm-associated monkey lymphomas and established cell lines from 3 of these tumors. Immunoblots of cell lysates were probed with polyspecific and monospecific reagents directed against human EB-virus EBNA1-6, and against the membrane protein LMP1. An EBNA2-cross-reacting protein was demonstrated in 8 lymphoma tissues (8/13) and in the 3 cell lines derived from the tumors. All tumors expressed a polypeptide with SO to 55 kDa molecular weight, which cross-reacted with some antibodies to EBNAI. Absorption experiments with normal monkey tissue showed that this polypeptide was specific for the cyno-EBV-carrying lymphoma cells. Equivalents of EBNA3-6 and LMP1 could not be detected. Immunophenotypical characterization showed that the monkey lymphomas were similar to human HIV-associated B-cell lymphomas. Malignant B-cell lymphomas in experimentally SIVsm-infected cynomolgus monkeys can be a model for EBV-associated lymphomagenesis in immunodeficiency states. (C) 1993 Wiley-Liss, Inc.