Prox1 expression in the endolymphatic sac revealed by whole-mount fluorescent imaging of Prox1-GFP transgenic mice
Prox1 expression in the endolymphatic sac revealed by whole-mount fluorescent imaging of Prox1-GFP transgenic mice
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DOI：
10.1016/j.bbrc.2014.12.068
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发表时间：
2015-01-30
影响因子：
3.1
通讯作者：
Peti-Peterdi, Janos
中科院分区：
文献类型：
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作者：
Miyashita, Takenori;Burford, James L.;Peti-Peterdi, Janos
这项研究描述了内淋巴囊研究的一项技术突破，这是通过使用最近产生的Prox1-GFP转基因小鼠模型实现的。通过脱钙的颞骨和对Prox1-GFP小鼠组织的三维观察，整体成像技术揭示了成人期内淋巴囊的阳性标记，并首次实现了基于GFP的内淋巴囊上皮细胞的鉴定。内淋巴囊上皮各部位均有PROX1的表达。在内淋巴囊的中间部分，富含线粒体的细胞不表达Prox1，尽管富含核糖体的细胞显示出很强的GFP标记。直观观察内淋巴囊与周围血管的解剖关系。在内淋巴囊中，Prox1的表达可能提示具有祖细胞样多能性或发育类似于其他器官中的系统淋巴管。这种内淋巴囊的整体成像技术可以与其他传统的组织学、切片和标记技术相结合，将对未来的内淋巴囊研究非常有用。(C)2014 Elsevier Inc.保留所有权利。
This study describes a technical breakthrough in endolymphatic sac research, made possible by the use of the recently generated Prox1-GFP transgenic mouse model. Whole-mount imaging techniques through the decalcified temporal bone and three-dimensional observations of Prox1-GFP mouse tissue revealed the positive labeling of the endolymphatic sac in adult stage, and allowed, for the first time, the GFP-based identification of endolymphatic sac epithelial cells. Prox1 expression was observed in all parts of the endolymphatic sac epithelia. In intermediate portion of the endolymphatic sac, mitochondria-rich cells did not express Prox1, although ribosome-rich cells showed strong GFP labeling. The anatomical relationship between the endolymphatic sac and the surrounding vasculature was directly observed. In the endolymphatic sac, expression of Prox1 may suggest progenitor cell-like pluripotency or developmental similarity to systemic lymphatic vessels in other organs. This whole-mount imaging technique of the endolymphatic sac can be combined with other conventional histological, sectioning, and labeling techniques and will be very useful for future endolymphatic sac research. (C) 2014 Elsevier Inc. All rights reserved.