The use of matK in Ophioglossaceae Phylogeny and the Determination of Mankyua Chromosome Number Shed Light on Chromosome Number Evolution in Ophioglossaceae
The use of matK in Ophioglossaceae Phylogeny and the Determination of Mankyua Chromosome Number Shed Light on Chromosome Number Evolution in Ophioglossaceae
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DOI：
10.1600/036364413x670232
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发表时间：
2013-10
期刊：
影响因子：
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通讯作者：
Wataru Shinohara;N. Nakato;Y. Yatabe-Kakugawa;T. Oka;Jong Kun Kim;N. Murakami;H. Noda;N. Sahashi
中科院分区：
文献类型：
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作者：
Wataru Shinohara;N. Nakato;Y. Yatabe-Kakugawa;T. Oka;Jong Kun Kim;N. Murakami;H. Noda;N. Sahashi
本文报道了与瓶舌草属（Ophioglossum s.l.）有着独特形态特征的单种厚囊属（Mankyua）的染色体数目和系统发育位置。和Helminthostachys，被确定。Mankyua的减数分裂和有丝分裂的染色体观察表明，n = 130和2n = ca。260，表明该属的碱基数为x = 130，这是一个新的发现，也是迄今为止报道的瓶舌科的最高碱基数。根据rbcL和matK基因的核苷酸序列变异构建的分子系统发育树在Mankyua的系统发育位置上存在差异。基于rbcL和matK数据集的分子系统发育树表明Mankyua可能是瓶舌草科最早分化的分支。
Abstract The chromosome number and phylogenetic position of the monotypic eusporangiate genus Mankyua (Ophioglossaceae), which shares peculiar morphological characteristics with Ophioglossum s.l. and Helminthostachys, were determined. Meiotic and mitotic chromosome observations of Mankyua show n = 130 and 2n = ca. 260, suggesting that the base number of this genus is x = 130, which is a new finding and the highest base number reported so far for Ophioglossaceae. The molecular phylogenetic trees derived from nucleotide sequence variation of rbcL and matK genes differ in terms of the phylogenetic position of Mankyua. The molecular phylogenetic trees based on combined data sets of rbcL and matK suggest that Mankyua might be the earliest-diverging lineage of Ophioglossaceae.