Histology after dural grafting with small intestinal submucosa
Histology after dural grafting with small intestinal submucosa
复制标题
DOI：
10.1016/s0090-3019(96)00202-9
复制
发表时间：
1996-10-01
影响因子：
--
通讯作者：
Boop, FA
中科院分区：
文献类型：
--
作者：
Cobb, MA;Badylak, SF;Boop, FA
背景技术由于可用材料具有显着的局限性，因此对理想的硬脑膜替代物的探索仍在继续。异种猪小肠粘膜下层(SIS)已成功用作多个身体器官系统的软组织移植物，评估其作为硬脑膜替代物的用途是合乎逻辑的。方法20只大鼠接受双半球去骨瓣联合硬脑膜切除术。在一侧进行 SIS 高嵌体移植。硬脑膜移植后7天和28天进行组织学评估，包括移植部位厚度、血管分布和细胞密度的描述性评估和定量评分。使用Student's t 检验比较各组的总分，p 值< 0.05 时接受显着性。 结果组织学评估显示移植物被梭形单核细胞浸润、结缔组织沉积和新血管形成。这种模式与先前描述的 SIS 移植物在其他部位的合并和重塑一致。在第 7 天（3.4 +/- 0.8 与 0.1 +/- 0.1）和第 28 天（4.6 +/- 1.1 与 2.2 +/- 0.5）时发现 SIS 移植部位和对照部位的组织学评分之间存在显着差异。没有观察到对底层皮质产生不利影响的证据。 结论 这项利用猪 SIS 作为硬脑膜替代品的初步研究结果是有希望的，因此证明了进一步长期研究的合理性。
BACKGROUND The search for the ideal dural substitute continues, inasmuch as available materials have significant limitations. Xenogeneic porcine small intestinal submucosa (SIS) has been successfully used as a soft tissue graft in several body organ systems, and it was logical to evaluate its use as a dural replacement.METHODS Twenty rats underwent bihemispheric craniectomy with dural resection. SIS onlay grafting on one side was performed. Histologic assessment was obtained at 7 and 28 days after dural grafting and included descriptive evaluation and quantitative scoring of graft-site thickness, vascularity, and cellular density. The total scores for the respective groups were compared using the Student's t test, significance being accepted for a p value < 0.05.RESULTS Histologic evaluation showed graft infiltration by spindle-shaped mononuclear cells, deposition of connective tissue, and neovascularity. This pattern is consistent with the previously described incorporation and remodeling of the SIS graft at other sites. A significant difference between the histologic scores of the SIS graft site and control site was found at 7 days (3.4 +/- 0.8 versus 0.1 +/- 0.1) and at 28 days (4.6 +/- 1.1 versus 2.2 +/- 0.5). No evidence of adverse effect on the underlying cortex was observed.CONCLUSIONS The results of this preliminary study utilizing porcine SIS as a dural substitute are promising and therefore justify further chronic studies.