FLAVIVIRUS INFECTION ENHANCEMENT IN MACROPHAGES - AN ELECTRON-MICROSCOPIC STUDY OF VIRAL CELLULAR ENTRY
FLAVIVIRUS INFECTION ENHANCEMENT IN MACROPHAGES - AN ELECTRON-MICROSCOPIC STUDY OF VIRAL CELLULAR ENTRY
复制标题
DOI：
10.1099/0022-1317-66-9-1969
复制
发表时间：
1985-01-01
影响因子：
3.8
通讯作者：
PORTERFIELD, JS
中科院分区：
文献类型：
--
作者：
GOLLINS, SW;PORTERFIELD, JS
采用同步感染的方法，在电镜水平上研究了西尼罗病毒（WNV）进入巨噬细胞样细胞系P388D1的方式。抗病毒单克隆抗体F6/16A的存在使病毒对P388D1细胞的附着增强了9倍，但对西尼罗病毒的进入途径没有影响。在F6/16A存在和不存在的情况下，单个病毒颗粒的初始摄取都是由包被的凹坑介导的，并在将细胞加热到37度的30秒内开始。C.病毒颗粒后来出现在完全或部分包被的囊泡中，后来出现在未包被的溶酶体前内吞液泡中，然后在溶酶体中降解。然而，病毒颗粒的聚集体（横截面上有5个或更多的病毒颗粒）似乎被细胞整体吞噬，这一过程涉及到聚集体被质膜的延伸所吞没。这一过程比单个病毒颗粒摄取的时间过程要慢，在将细胞加热到37度后15至30分钟变得突出。C.酸性磷酸酶染色失败表明溶酶体前体空泡室参与进入过程。当病毒内化发生在20度时，这个隔室可以特异性地装载病毒颗粒。C在50mm氯化铵存在下。
The mode of entry of West Nile virus (WNV) into the macrophage-like cell line P388D1 was investigated at the electron microscopical level using synchronized infections. The presence of the antiviral monoclonal antibody F6/16A at a concentration that enhanced viral attachment to P388D1 cells ninefold made no difference to the entry pathway of WNV. In both the absence and presence of F6/16A the initial uptake of single viral particles was mediated by coated pits, and started within 30 s of warming the cells to 37.degree. C. Viral particles later appeared in fully or partially coated vesicles and later in uncoated prelysosomal endocytic vacuoles before degradation in lysosomes. However, aggregates of viral particles (five or more virus particles in cross-section), appeared to be phagocytosed whole by cells in a process which involved aggregates being engulfed by extensions of the plasma membrane. This process exhibited a slower time course than the uptake of single viral particles, becoming prominent 15 to 30 min after warming the cells to 37.degree. C. The involvement of a prelysosomal vacuolar compartment in the entry process was shown by a failure to stain for acid phosphatase. This compartment could be specifically loaded with viral particles when viral internalization occurred at 20.degree. C in the presence of 50 mM-ammonium chloride.