Oil-Membrane Protection of Electrochemical Sensors for Fouling- and pH-Insensitive Detection of Lipophilic Analytes
Oil-Membrane Protection of Electrochemical Sensors for Fouling- and pH-Insensitive Detection of Lipophilic Analytes
复制标题
用于亲脂性分析物的结垢和 pH 不敏感检测的电化学传感器的油膜保护
DOI：
10.1021/acsami.1c14175
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发表时间：
2021
影响因子：
9.5
通讯作者：
Heikenfeld, Jason
中科院分区：
文献类型：
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作者：
Yuan, Yuchan;DeBrosse, Madeleine;Brothers, Michael;Kim, Steve;Sereda, Alexandra;Ivanov, Nikolai V.;Hussain, Saber;Heikenfeld, Jason
为了充分利用电化学传感器的免试剂和免标记传感能力，一个经常且仍然存在的挑战是，由于样品溶液中不受控制的pH或盐度或样品溶液中的污物，传感器会受到干扰和降解。在这里，我们提出了一种油膜传感器保护技术，允许疏水(亲脂)分析物渗透到包含理想盐度和pH条件的密封传感器舱中，同时阻止常见的亲水性干扰物(蛋白质、酸、碱等)。在本文中，我们通过演示基于电化学适配子(EAB)传感器的皮质醇连续检测来验证油膜传感器保护技术。封装的EAB皮质醇传感器的浓度上升时间为5分钟，即使在pH值为3的酸性溶液的极端条件下，也能保持至少7小时的测量信号。
To take full advantage of the reagent- and label-free sensing capabilities of electrochemical sensors, a frequent and remaining challenge is interference and degradation of the sensors due to uncontrolled pH or salinity in the sample solution or foulants from the sample solution. Here, we present an oil-membrane sensor protection technique that allows for the permeation of hydrophobic (lipophilic) analytes into a sealed sensor compartment containing ideal salinity and pH conditions while simultaneously blocking common hydrophilic interferents (proteins, acids, bases, etc.) In this paper, we validate the oil-membrane sensor protection technique by demonstrating continuous cortisol detection via electrochemical aptamer-based (EAB) sensors. The encapsulated EAB cortisol sensor exhibits a 5 min concentration-on rise time and maintains a measurement signal of at least 7 h even in the extreme condition of an acidic solution of pH 3.