Systemic and mucosal immunity in mice elicited by a single immunization with human adenovirus type 5 or 41 vector-based vaccines carrying the spike protein of Middle East respiratory syndrome coronavirus.
Systemic and mucosal immunity in mice elicited by a single immunization with human adenovirus type 5 or 41 vector-based vaccines carrying the spike protein of Middle East respiratory syndrome coronavirus.
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作者：
Guo X;Deng Y;Chen H;Lan J;Wang W;Zou X;Hung T;Lu Z;Tan W
针对粘膜病原体如中东呼吸综合征冠状病毒（MERS-CoV）的理想疫苗应在全身和粘膜水平上提供持续的保护性免疫。在这里，我们评估了通过单次肌内或胃内给予表达MERS-CoV刺突蛋白的重组腺病毒5型（Ad 5）或41型（Ad 41）疫苗诱导的体内全身和粘膜抗原特异性免疫应答。Ad 5-S或Ad 41-S的灌胃给药诱导血清中的抗原特异性IgG和中和抗体;然而，未检测到抗原特异性T细胞应答。相反，在单次肌肉注射Ad 5-S或Ad 41-S后，在小鼠的脾和肺淋巴细胞中引起功能性抗原特异性T细胞应答，并持续数月。两种基于rAd的疫苗经肌肉内施用均诱导全身体液免疫应答（中和IgG抗体）。我们的研究结果表明，单剂量的Ad 5-S-或Ad 41-S-为基础的疫苗代表了一个有吸引力的策略，用于控制MERS-CoV感染和传播。
An ideal vaccine against mucosal pathogens such as Middle East respiratory syndrome coronavirus (MERS-CoV) should confer sustained, protective immunity at both systemic and mucosal levels. Here, we evaluated the in vivo systemic and mucosal antigen-specific immune responses induced by a single intramuscular or intragastric administration of recombinant adenoviral type 5 (Ad5) or type 41 (Ad41) -based vaccines expressing the MERS-CoV spike (S) protein. Intragastric administration of either Ad5-S or Ad41-S induced antigen-specific IgG and neutralizing antibody in serum; however, antigen-specific T-cell responses were not detected. In contrast, after a single intramuscular dose of Ad5-S or Ad41-S, functional antigen-specific T-cell responses were elicited in the spleen and pulmonary lymphocytes of the mice, which persisted for several months. Both rAd-based vaccines administered intramuscularly induced systemic humoral immune responses (neutralizing IgG antibodies). Our results show that a single dose of Ad5-S- or Ad41-S-based vaccines represents an appealing strategy for the control of MERS-CoV infection and transmission.