Study of solvent–protein coupling effects by neutron scattering
Study of solvent–protein coupling effects by neutron scattering
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通过中子散射研究溶剂-蛋白质耦合效应
DOI：
10.1007/s10867-009-9177-5
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发表时间：
2010
影响因子：
1.8
通讯作者：
M. Telling
中科院分区：
文献类型：
--
作者：
Balázs Varga;F. Migliardo;F. Migliardo;E. Takács;Beáta G. Vértessy;S. Magazù;M. Telling
目前的工作旨在表征浸入生物保存液体和玻璃中的蛋白质的动态行为。为此，选择了蛋白质 dUTPase，而所选溶剂为甘油、三醇和一些同源二糖，即海藻糖、麦芽糖和蔗糖，已知它们是非常有效的生物防腐剂。结果强调，二糖对水动力学表现出减缓作用，海藻糖的这种作用比其他二糖更强。此外，通过使用基于弹性非相干中子散射提取的均方位移的易碎性的有效定义来表征承载蛋白质的介质，这与基于粘度的 Angell 动力学易碎性直接相关。最后，对溶剂中隔离的蛋白质的动力学进行了研究。结果表明蛋白质动力学与周围基质的动力学耦合。
The present work aims to characterize the dynamical behavior of proteins immersed in bio-preserving liquids and glasses. For this purpose, the protein dUTPase was chosen, while the selected solvents were glycerol, a triol, and some homologous disaccharides, i.e., trehalose, maltose, and sucrose, which are known to be very effective bio-preserving agents. The results highlight that the disaccharides show a slowing down effect on the water dynamics, which is stronger for trehalose than in the case of the other disaccharides. Furthermore, a characterization of the medium which hosts the protein is performed by using an operative definition of fragility based on the mean square displacement extracted by elastic incoherent neutron scattering, which is directly connected to Angell’s kinetic fragility based on the viscosity. Finally, a study of the dynamics of the protein sequestered within the solvents is performed. The result shows that the protein dynamics is coupled with that of the surrounding matrix.