Cell surface Lactobacillus plantarum LA 318 glyceraldehyde-3-phosphate dehydrogenase (GAPDH) adheres to human colonic mucin
Cell surface Lactobacillus plantarum LA 318 glyceraldehyde-3-phosphate dehydrogenase (GAPDH) adheres to human colonic mucin
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DOI：
10.1111/j.1365-2672.2007.03679.x
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发表时间：
2008-06-01
影响因子：
4
通讯作者：
Saito, T.
中科院分区：
文献类型：
--
作者：
Kinoshita, H.;Uchida, H.;Saito, T.
目的：鉴定植物乳杆菌LA 318对人结肠粘蛋白(HCM)的高粘附性。方法：粘附性试验采用Biaccore法，PBS洗涤的细菌细胞对HCM的粘附率明显低于蒸馏水洗的细菌细胞。PBS洗涤组分中的一个组分附着在HCM上，用SDS-PAGE检测到一个主要蛋白质为一条约40-kDa的条带。通过N端氨基酸序列与序列数据库的同源性比较，该蛋白被鉴定为甘油醛-3-磷酸脱氢酶(GAPDH)。结果表明，LA 318的GAPDH与植物乳杆菌WCFS1的GAPDH(GapB)同源性为100%。结论：我们在植物乳杆菌LA 318的PBS洗脱液中发现了其对HCM的粘附素。经鉴定，该分子为GAPDH。由于LA 318与许多病原体具有相同的粘附素，因此乳酸杆菌GAPDH可能与感染肠道的病原体竞争。研究的意义和影响：这是第一份报告显示表达在乳杆菌细胞表面的GAPDH与粘蛋白附着，表明植物乳杆菌LA 318通过GAPDH结合活性附着于HCM，从而定植于人的肠道粘膜。
Aims: To characterize the adhesion molecule of Lactobacillus plantarum LA 318 that shows high adhesion to human colonic mucin (HCM).Methods: The adhesion test used the BIACORE assay where PBS-washed bacterial cells showed a significant decrease in adherence to HCM than distilled water-washed cells. A component in the PBS wash fraction adhered to the HCM and a main protein was detected as a c. 40-kDa band using SDS-PAGE. Using homology comparisons of the N-terminal amino acid sequences compared with sequence databases, this protein was identified as glyceraldehyde-3-phosphate dehydrogenase (GAPDH). The DNA sequence of LA 318 GAPDH was 100% identical to the GAPDH (gapB) of L. plantarum WCFS1. The purified GAPDH adhered to HCM.Conclusions: We found the adhesin of L. plantarum LA 318 to HCM in its culture PBS wash fraction. The molecule was identified as GAPDH. Because LA 318 possesses the same adhesin as many pathogens, the lactobacilli GAPDH may compete with pathogens infecting the intestine.Significance and Impact of the Study: This is the first report showing GAPDH expressed on the cell surface of lactobacilli adheres to mucin suggesting L. plantarum LA 318 adheres to HCM using GAPDH binding activity to colonize the human intestinal mucosa.