Rapid mixing methods for exploring the kinetics of protein folding
Rapid mixing methods for exploring the kinetics of protein folding
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DOI：
10.1016/j.ymeth.2004.03.003
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发表时间：
2004-09-01
期刊：
影响因子：
4.8
通讯作者：
Shastry, MCR
中科院分区：
文献类型：
--
作者：
Roder, H;Maki, K;Shastry, MCR
关于分子种类的时间依赖性的信息对于阐明化学和生物学中的反应机理是至关重要的。涉及两种或更多种溶液的湍流混合的快速流动实验仍然是蛋白质折叠和其他生物化学过程（如配体结合和酶促反应）的动力学信息的主要来源。混合器设计和检测方法的最新进展为在微秒时间尺度上探索蛋白质的构象变化打开了新的窗口。这些进展对于探索蛋白质折叠的早期阶段尤其重要。(C)2004年爱思唯尔公司All rights reserved.
Information on the time-dependence of molecular species is critical for elucidating reaction mechanisms in chemistry and biology. Rapid flow experiments involving turbulent mixing of two or more solutions continue to be the main source of kinetic information on protein folding and other biochemical processes, such as ligand binding and enzymatic reactions. Recent advances in mixer design and detection methods have opened a new window for exploring conformational changes in proteins on the microsecond time scale. These developments have been especially important for exploring early stages of protein folding. (C) 2004 Elsevier Inc. All rights reserved.