RUTHENIUM RED INHIBITS THE MITOCHONDRIAL CA-2+ UPTAKE IN INTACT BOVINE SPERMATOZOA AND INCREASES THE CYTOSOLIC CA2+ CONCENTRATION
RUTHENIUM RED INHIBITS THE MITOCHONDRIAL CA-2+ UPTAKE IN INTACT BOVINE SPERMATOZOA AND INCREASES THE CYTOSOLIC CA2+ CONCENTRATION
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DOI：
10.1016/0014-5793(86)81193-0
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发表时间：
1986-03-17
期刊：
影响因子：
3.5
通讯作者：
DEANA, R
中科院分区：
文献类型：
--
作者：
RIGONI, F;DEANA, R
射精牛精子对Ca2+的吸收和循环几乎被钌红、抗霉素A或FCCP完全消除。在洗涤染料预处理的细胞并添加洋地黄皂苷或菲律宾素后，也观察到钌红的抑制作用。相反，A23187 治疗克服了抑制作用。结论是，钌红渗透到完整的精子中，“原位”抑制线粒体 Ca2+ 摄取，并导致胞质游离 Ca2+ 浓度明显增加。
The uptake and cycling of Ca2+by ejaculated bovine spermatozoa are almost completely abolished by ruthenium red, antimycin A or FCCP. The inhibitory effect of ruthenium red is also observed after washing of the dye‐pretreated cells followed by addition of digitonin or filipin. In contrast, the inhibition is overcome by A23187 treatment. It is concluded that ruthenium red penetrates into intact spermatozoa, inhibits the mitochondrial Ca2+uptake ‘in situ’, and causes the observed increase of the cytosolic free Ca2+concentration.