Molecular cloning, expression, and characterization of angiopoietin-related protein - Angiopoietin-related protein induces endothelial cell sprouting
Molecular cloning, expression, and characterization of angiopoietin-related protein - Angiopoietin-related protein induces endothelial cell sprouting
复制标题
DOI：
10.1074/jbc.274.37.26523
复制
发表时间：
1999-09-10
影响因子：
4.8
通讯作者：
Koh, GY
中科院分区：
文献类型：
--
作者：
Kim, I;Moon, SO;Koh, GY
利用简并聚合酶链反应，我们从人和小鼠成体心脏cDNA中分离到一个编码493个氨基酸的新蛋白的cDNA，并将其命名为血管生成素相关蛋白2（ARP2）。ARP2的NH 2末端和COOH末端部分含有血管生成素中保守的特征性卷曲螺旋结构域和纤维蛋白原样结构域，ARP2具有两个共有糖基化位点和在NH1末端的高度疏水区域，其是典型的分泌信号序列。在COS细胞中表达的重组ARP 2是分泌型的，糖基化的。在成人组织中，ARP 2 mRNA在心脏、小肠、脾和胃中最丰富。在大鼠胚胎中，ARP2 mRNA在血管和骨骼肌中最丰富。内皮细胞和血管平滑肌细胞也含有ARP 2 mRNA，重组ARP 2蛋白诱导血管内皮细胞出芽，但不与蒂尔或TieB受体结合。这些结果表明，ARP2可能通过自分泌或旁分泌作用对内皮细胞发挥作用。
Using degenerate polymerase chain reaction, we isolated a cDNA encoding a novel 493-amino acid protein from human and mouse adult heart cDNAs and have designated it angiopoietin-related protein-2 (ARP2), The NH2-terminal and COOH-terminal portions of ARP2 contain the characteristic coiled-coil domain and fibrinogen-like domain that are conserved in angiopoietins, ARP2 has two consensus glycosylation sites and a highly hydrophobic region at the NH, terminus that is typical of a secretory signal sequence. Recombinant ARP2 expressed in COS cells is secreted and glycosylated, In human adult tissues, ARP2 mRNA is most abundant in heart, small intestine, spleen, and stomach. In rat embryos, ARP2 mRNA is most abundant in the blood vessels and skeletal muscles. Endothelial and vascular smooth muscle cells also contain ARP2 mRNA, Recombinant ARP2 protein induces sprouting in vascular endothelial cells but does not bind to the Tiel or TieB receptor. These results suggest that ARP2 may exert a function on endothelial cells through autocrine or paracrine action.