Controlling caloric consumption: protocols for rodents and rhesus monkeys
Controlling caloric consumption: protocols for rodents and rhesus monkeys
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DOI：
10.1016/s0197-4580(99)00043-3
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发表时间：
1999-03-01
影响因子：
4.2
通讯作者：
Weindruch, R
中科院分区：
文献类型：
--
作者：
Pugh, TD;Klopp, RG;Weindruch, R
研究生物衰老的一种方法是将对照喂养的动物与其他限制卡路里摄入量20%或更多的动物进行比较。热量限制（CR）是唯一的干预措施，可以延长几种无脊椎动物和脊椎动物的最大寿命，包括蜘蛛，鱼，大鼠和小鼠。CR延缓非人灵长类动物衰老的能力目前正在探索中。啮齿动物研究表明，CR反对许多与年龄相关的病理生理变化的发展，包括大脑的变化以及学习和行为的变化。在啮齿动物中研究CR的一个目标是确定它延缓衰老的机制，以设计复制这些效果的干预措施。我们使用的方法进行CR研究的小鼠和恒河猴的描述。我们采用的程序旨在实现研究中所有动物的高度热量控制。如在我们的研究中所使用的，该对照包括以下特征：1）动物被单独圈养，以及2）对照组中的所有个体食用相同数量的卡路里（即，它们不是随意进食的）。尽管该方法对所有动物进行了严格的热量控制，但成功进行CR研究似乎具有相当大的程序灵活性。(C)1999 Elsevier Science Inc. All rights reserved.
One approach for investigating biological aging is to compare control-fed animals with others restricted in calorie intake by 20% or more. Caloric restriction (CR) is the only intervention shown to extend the maximum lifespan of several invertebrates and vertebrates including spiders, fish, rats and mice. The capacity of CR to retard aging in nonhuman primates is now being explored. The rodent studies show that CR opposes the development of many age-associated pathophysiological changes, including changes to the brain and changes in learning and behavior. One goal of studying CR in rodents is to determine the mechanisms by which it retards aging to design interventions that duplicate those effects. The methods that we use for conducting CR studies on mice and rhesus monkeys are described. We employ procedures designed to achieve a high degree of caloric control for all animals in the study. As used in our studies, this control includes the following features: 1) animals are individually housed, and 2) all individuals in the control group eat the same number of calories (i.e., they are not fed ad lib). Although this method results in strict caloric control for all animals, there seems to be considerable procedural flexibility for the successful conduct of CR studies. (C) 1999 Elsevier Science Inc. All rights reserved.