Global analyses of the dynamics of mammalian microRNA metabolism
Global analyses of the dynamics of mammalian microRNA metabolism
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DOI：
10.1101/gr.251421.119
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发表时间：
2019-11-01
期刊：
影响因子：
7
通讯作者：
Bartel, David P.
中科院分区：
文献类型：
--
作者：
Kingston, Elena R.;Bartel, David P.
生产和降解的速度共同决定了microRNA （miRNA）的丰度和动态。在这里，我们使用稳态代谢标记方法来评估接触抑制小鼠胚胎成纤维细胞（mef）中的176种mirna，分裂mef中的182种mirna和小鼠胚胎干细胞（mESCs）中的127种mirna的这些速率。MicroRNA双链，每个包括一个成熟的miRNA及其客运链，以110 +/- 50拷贝/细胞/分钟的速度产生，超过了任何mrna的报道速率。这些复合体被迅速装入阿尔戈瑙特
Rates of production and degradation together specify microRNA (miRNA) abundance and dynamics. Here, we used approach-to-steady-state metabolic labeling to assess these rates for 176 miRNAs in contact-inhibited mouse embryonic fibroblasts (MEFs), 182 miRNAs in dividing MEFs, and 127 miRNAs in mouse embryonic stem cells (mESCs). MicroRNA duplexes, each comprising a mature miRNA and its passenger strand, are produced at rates as fast as 110 +/- 50 copies/cell/min, which exceeds rates reported for any mRNAs. These duplexes are rapidly loaded into Argonaute, with