Distinct and overlapping roles of single-repeat MYB genes in root epidennal patterning
Distinct and overlapping roles of single-repeat MYB genes in root epidennal patterning
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DOI：
10.1016/j.ydbio.2007.09.001
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发表时间：
2007-11-15
影响因子：
2.7
通讯作者：
Schiefelbein, John
中科院分区：
文献类型：
--
作者：
Simon, Marissa;Lee, Myeong Min;Schiefelbein, John
拟南芥根表皮的细胞分化形成了根毛细胞和非毛细胞的位置依赖模式。本文对单重复R3 MYB基因家族的5个成员，包括CAPRICE （CPC）、tritychon （TRY）、tryenhancer of TRY和CPC 1、2和3 （ETC1、ETC2和ETC3）进行了全面分析，并研究了它们在根表皮细胞分化中的作用和功能关系。遗传和表达分析表明，CPC、try和ETC1通过抑制非毛特异性来促进毛细胞的命运，而ETC2和ETC3则没有。此外，我们发现单重复MYB活性在整个根发育过程中是必需的，从胚胎发生开始。我们还发现了一种新的调控相互作用，其中GLABRA2 （GL2）促进根表皮中TRY（但不包括CPC或ETC）的表达，从而产生第二个侧抑制反馈回路。将CPC调控元件与每个单重复MYB基因的蛋白质编码区结合的基因融合实验表明，尽管TRY和ETC2与CPC/ETC1/ETC3存在差异，但这五种蛋白在功能上是相似的。这些结果为这些单重复myb的功能提供了新的见解，并表明它们的调控序列的差异在很大程度上负责它们在表皮细胞模式中的不同作用。(c) 2007爱思唯尔公司版权所有。
Cell specification in the root epidermis of Arabidopsis generates a position-dependent pattern of root-hair cells and non-hair cells. Here we conduct a comprehensive analysis of the five members of a single-repeat R3 MYB gene family, including CAPRICE (CPC, TRIPTYCHON (TRY), ENHANCER of TRYand CPC 1, 2, and 3 (ETC1, ETC2, and ETC3), and study their role and functional relationship in root epidermal cell specification. Based on genetic and expression analyses, CPC, TRYand ETC1, but not ETC2 or ETC3, promote the hair cell fate by inhibiting non-hair specification. Further, we find that single-repeat MYB activity is required for epidennal patterning throughout root development, beginning during embryogenesis. We also identify a novel regulatory interaction whereby GLABRA2 (GL2) promotes TRY (but not CPC or ETC]) expression in the root epidermis, which generates a second lateral inhibition feedback loop. Gene fusion experiments combining CPC regulatory elements with protein-coding regions of each single-repeat MYB gene suggest that all five proteins are functionally similar, although TRY and ETC2 exhibit distinctions from CPC/ETC1/ETC3. These results provide new insight into the function of these single-repeat MYBs and suggest that divergence of their regulatory sequences is largely responsible for their distinct roles in epidermal cell patterning. (c) 2007 Elsevier Inc. All rights reserved.