In vitro antibody response to tetanus in the MIMIC™ system is a representative measure of vaccine immunogenicity
In vitro antibody response to tetanus in the MIMIC™ system is a representative measure of vaccine immunogenicity
复制标题
DOI：
10.1016/j.biologicals.2009.02.018
复制
发表时间：
2009-06-01
期刊：
影响因子：
1.7
通讯作者：
Wittman, Vaughan
中科院分区：
文献类型：
--
作者：
Byers, Anthony M.;Tapia, Tenekua M.;Wittman, Vaughan
为了应对动物疫苗保护研究一再未能准确预测人体试验结果的情况，我们开发了一种完全人类模块免疫体外构建物(MIMIC(TM))，作为潜在疫苗配方有效性测试的初步筛选。为了验证这种方法的潜力，我们在接受破伤风疫苗接种之前和之后监测了一组捐赠者体内体外产生的破伤风(TT)特异性抗体水平。将纯化的T细胞和B细胞与自身破伤风疫苗致敏的树突状细胞结合，产生特异性抗体。ELISPOT对TT特异性抗体分泌细胞的计数显示，接种疫苗后这一群体的数量显著增加。免疫前后体外产生的TT特异性抗体反应的相对大小在很大程度上概括了在同一个体中测量的TT特异性IgG血清滴度分布。这些发现提供了证据，证明MIMIC(TM)系统可以是一种快速和具有代表性的体外方法，通过诱导记忆B细胞反应来测量疫苗的免疫原性。(C)2009年国际生物制品协会。爱思唯尔有限公司出版。保留所有权利。
In response to the recurrent failure of animal vaccine protection studies to accurately predict human trial results, we have developed a fully human modular immune in vitro construct (MIMIC (TM)) to serve as a preliminary screen for efficacy testing of potential vaccine formulations. To validate the potential of this approach, we monitored the in vitro-generated tetanus (TT)-specific antibody levels in a cohort of donors before and after receiving tetanus vaccination. Purified CD4_T cell and B cell populations were combined with autologous tetanus vaccine-pulsed dendritic cells to generate specific antibody. Enumeration of TT-specific IgG antibody-secreting cells by ELISPOT displayed a significant increase in the magnitude of this population after vaccination. The relative magnitudes of the in vitro-generated TT-specific antibody response before and after vaccination largely recapitulated the TT-specific IgG serum titer profiles measured in the same individuals. These findings provide evidence that the MIMIC (TM) system can be a rapid and representative in vitro method for measuring vaccine immunogenicity via induction of the memory B cell response. (C) 2009 The International Association for Biologicals. Published by Elsevier Ltd. All rights reserved.