Cytokine effects on pepsinogen secretion from human peptic cells.
Cytokine effects on pepsinogen secretion from human peptic cells.
复制标题
细胞因子对人消化细胞分泌胃蛋白酶原的影响。
作者：
M. Serrano;A. Lanas;S. Lorente;R. Sáinz
背景：不同的细胞因子，包括表皮生长因子（EGF）和白细胞介素1β（IL 1β），通过旁分泌或自分泌的不同机制参与胃粘膜损伤和修复的发病机制。目的：研究EGF和IL 1β是否影响体外胃蛋白酶原分泌。方法：通过内窥镜获得的活检标本经胶原酶消化、机械破碎和密度梯度离心后制备分散的人消化细胞。结果：EGF 剂量依赖性地增加基础胃蛋白酶原分泌和 EGF 诱导的次最大刺激的促有丝分裂浓度 (0.1 nM)。使用转化生长因子α观察到类似的效果。 EGF 对胃蛋白酶原分泌的影响是除了 CCK-OP 和 db-cAMP 刺激的胃蛋白酶原分泌所诱导的影响之外。 EGF 刺激的胃蛋白酶原分泌被人类免疫特异性 EGF 受体抗体完全抑制，并被两种不同的酪氨酸激酶抑制剂金雀异黄素和酪氨酸磷酸酶 25 减少。 IL 1 beta 不影响基础、CCK-OP 或乙酰胆碱刺激的胃蛋白酶原分泌。然而，IL 1β 剂量依赖性地抑制 db-cAMP 和组胺刺激的胃蛋白酶原分泌。结论：这些结果表明EGF和IL 1β在体外调节人胃蛋白酶原分泌，并表明这些细胞因子对胃蛋白酶原分泌的旁分泌作用可能与胃粘膜损伤和炎症的某些病理状况有关。
BACKGROUND: Different cytokines, including epidermal growth factor (EGF) and interleukin 1 beta (IL 1 beta), participate in the pathogenesis of gastric mucosal damage and repair by means of different mechanisms that are either paracrine or autocrine in nature. AIMS: To study whether EGF and IL 1 beta affect pepsinogen secretion in vitro. METHODS: Dispersed human peptic cells were prepared from endoscopically obtained biopsy specimens after collagenase digestion, mechanical disruption, and density gradient centrifugation. RESULTS: EGF dose dependently increased basal pepsinogen secretion and mitogenic concentrations (0.1 nM) of EGF induced submaximal stimulation. Similar effects were observed with transforming growth factor alpha. EGF effects on pepsinogen secretion were in addition to that induced by CCK-OP and db-cAMP stimulated pepsinogen secretion. EGF stimulated pepsinogen secretion was completely inhibited by a human immunospecific EGF receptor antibody and reduced by both genistein and tyrphostin-25, two different tyrosine kinase inhibitors. IL 1 beta does not affect basal, CCK-OP or acetylcholine stimulated pepsinogen secretion. However, IL 1 beta dose dependently inhibited db-cAMP and histamine stimulated pepsinogen secretion. CONCLUSIONS: These results show that both EGF and IL 1 beta modulate human pepsinogen secretion in vitro and suggest that the paracrine effects of these cytokines on pepsinogen secretion might be involved in some pathological conditions of damage and inflammation of the gastric mucosa.
重组白介素-1 抑制大鼠胃酸分泌的中枢作用。
影响因子：
29.4
作者：
Saperas,ES;Yang,H;Rivier,C;Taché,Y
通讯作者：
Taché,Y
影响因子：
11.2
作者：
H. Masui;T. Kawamoto;J. Sato;B. Wolf;G. Sato;J. Mendelsohn
通讯作者：
H. Masui;T. Kawamoto;J. Sato;B. Wolf;G. Sato;J. Mendelsohn
酪氨酸激酶活性调节刺激的壁细胞酸分泌。
DOI：
10.1152/ajpgi.1993.264.2.g351
发表时间：
1993
期刊：
The American journal of physiology
影响因子：
--
作者：
Tsunoda,Y;Modlin,IM;Goldenring,JR
通讯作者：
Goldenring,JR