Enoxacin-loaded Poly (lactic-co-glycolic acid) Coating on Porous Magnesium Scaffold as a Drug Delivery System: Antibacterial Properties and Inhibition of Osteoclastic Bone Resorption
Enoxacin-loaded Poly (lactic-co-glycolic acid) Coating on Porous Magnesium Scaffold as a Drug Delivery System: Antibacterial Properties and Inhibition of Osteoclastic Bone Resorption
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多孔镁支架上负载依诺沙星的聚乳酸-乙醇酸涂层作为药物输送系统：抗菌特性和破骨骨吸收抑制
DOI：
10.1016/j.jmst.2016.07.013
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发表时间：
2016
影响因子：
10.9
通讯作者：
Dai Kerong
中科院分区：
文献类型：
--
作者：
Li Yang;Liu Xuqiang;Tan Lili;Ren Ling;Wan Peng;Hao Yongqiang;Qu Xinhua;Yang Ke;Dai Kerong
植入物相关感染仍然是骨科手术中的一个难题。因此，开发用于治疗感染和再生可能由感染引起的骨组织损失的多功能骨植入物是重要的。本研究联合收割机镁的优良性质、依诺沙星的抗菌性能和抑制骨吸收的作用，制备了载依诺沙星的聚乳酸-羟基乙酸共聚物（PLGA）多孔镁支架（Enox-PLGA-Mg）。Mg支架的载药PLGA涂层使得载药效率（52%-56%）高于未涂层的载依诺沙星的Mg支架（Enox-Mg）（4%-5%）。Enox-PLGA-Mg具有超过14天的持续药物释放，并且这种依诺沙星的受控释放显著抑制细菌粘附并防止表皮葡萄球菌（ATCC 35984）和金黄色葡萄球菌（ATCC 25923）形成生物膜。Balb/c小鼠胚胎成纤维细胞（Balb/c 3 T3细胞）的生物相容性测试表明PLGA-Mg比Mg具有更好的生物相容性。最后，我们还证明了Enox-PLGA-Mg提取物在体外有效地抑制破骨细胞的形成。因此，Enox-PLGA-Mg有可能用作多功能可控药物递送系统骨支架，以预防和/或治疗骨科植入物周围感染。
Implant-associated infection remains a difficult medical problem in orthopedic surgery. Therefore, the development of multifunctional bone implants for treating infection and regenerating lost bone tissue, which may be a result of infection, is important. In the present study, we report the fabrication of enoxacin-loaded poly (lactic-co-glycolic acid) (PLGA) coating on porous magnesium scaffold (Enox-PLGA-Mg) which combine the favorable properties of magnesium, the antibacterial property and the effect of inhibition of osteoclastic bone resorption of enoxacin. The drug loaded PLGA coating of Mg scaffold enables higher drug loading efficiency (52%–56%) than non-coating enoxacin loaded Mg scaffold (Enox-Mg) (4%–5%). Enox-PLGA-Mg exhibits sustained drug release for more than 14 days, and this controlled release of enoxacin significantly inhibits bacterial adhesion and prevented biofilm formation byStaphylococcus epidermidis(ATCC35984) andStaphylococcus aureus(ATCC25923). Biocompatibility tests with Balb/c mouse embryo fibroblasts (Balb/c 3T3 cells) indicate that PLGA-Mg has better biocompatibility than Mg. Finally, we also demonstrate that Enox-PLGA-Mg extract potently inhibited osteoclast formationin vitro. Therefore, Enox-PLGA-Mg has the potential to be used as a multifunctional controlled drug delivery system bone scaffolds to prevent and/or treat orthopedic peri-implant infections.