Analysis of DLC-1 expression in human breast cancer
Analysis of DLC-1 expression in human breast cancer
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DOI：
10.1007/s00432-003-0440-z
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发表时间：
2003-06-01
影响因子：
3.6
通讯作者：
Scherneck, S
中科院分区：
文献类型：
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作者：
Plaumann, M;Seitz, S;Scherneck, S
染色体区域8 p12-p22在包括乳腺癌（BC）在内的许多肿瘤中显示频繁的等位基因丢失。DLC-1基因定位于8 p21-p22，可能是该区域的候选抑癌基因。为了评估DLC-1在乳腺癌发生中的参与，我们研究了DLC-1 mRNA在一组14个原代人BC和相应的正常乳腺细胞以及8个BC细胞系中的表达。在57%的原代BC和62.5%的BC细胞系中分别观察到低水平或不存在DLC-1 mRNA。我们没有发现DLC-1基因座缺失与DLC-1 mRNA表达之间存在任何相关性。将该基因转染到DLC-1缺陷型T-47 D细胞中，DLC-1 mRNA水平升高，并导致细胞生长抑制和集落形成能力降低。我们的研究结果表明DLC-1在BC致癌作用中的作用。
The chromosome region 8p12-p22 shows frequent allelic loss in many neoplasms, including breast cancer (BC). The DLC-1 gene, located on 8p21-p22, might be a candidate tumor suppressor gene in this region. To evaluate the involvement of DLC-1 in breast carcinogenesis we studied DLC-1 mRNA expression in a panel of 14 primary human BC and the corresponding normal breast cells as well as 8 BC cell lines. Low levels or absence of DLC-1 mRNA were observed in 57% of primary BC and 62.5% of BC cell lines, respectively. We could not find any correlation between DLC-1mRNA expression and deletions at the DLC-1 locus. Transfection of the gene into DLC-1 deficient T-47D cells raised the DLC-1 mRNA level and resulted in inhibition of cell growth and reduced colony-forming capacity. Our results indicate a role of DLC-1 in BC carcinogenesis.