Chondrogenic stimulation in mesenchymal stem cells using scaffold-based sustained release of platelet-rich plasma
Chondrogenic stimulation in mesenchymal stem cells using scaffold-based sustained release of platelet-rich plasma
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DOI：
10.1002/app.50075
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发表时间：
2020-10-23
影响因子：
3
通讯作者：
Fakhrzadeh, Hossein
中科院分区：
文献类型：
--
作者：
Bolandi, Behzad;Imani, Rana;Fakhrzadeh, Hossein
高度期望通过可注射水凝胶开发用于有效修复软骨缺损的微创策略。可注射水凝胶可同时包埋细胞和生长因子（GF），可作为原位形成的支架，并可支持加速的组织再生过程。本研究的目的是制备一种基于藻酸盐（Alg）/聚乙烯醇（PVA）的复合可注射水凝胶，并将富血小板血浆（PRP）包裹的藻酸盐硫酸盐（AlgS）微球作为GFs的局部缓释系统，用于关节软骨再生。结果表明，合成的AlgS微球支持PRP GFs在14天内的持续释放，其中保留了它们的生物活性比游离PRP更多。兔脂肪间充质干细胞与负载PRP的AlgS珠接触显示出更多的增殖（2.7倍），并且具有比游离PRP处理的细胞明显更高的I型胶原和GAG沉积。此外，与PRP直接处理相比，包封在包括PRP持续释放系统的水凝胶中的细胞显示出作为软骨关键基因的胶原类型Iota Iota（61倍）、聚集蛋白聚糖（294倍）和SOX 9（71.5倍）的上调表达。总之，开发的混合Alg/PVA水凝胶包埋与PRP封装的AlgS微珠被建议作为一个潜在的原位形成的软骨再生支架。
Developing minimal invasive strategies via injectable hydrogels for effective repairing of cartilage defects is highly desired. Injectable hydrogels, which can simultaneously embed cell and growth factors (GFs), serve as in situ formed scaffolds and could support an accelerated tissue regeneration process. The purpose of this study is to fabricate a composite injectable hydrogel, based on alginate (Alg)/polyvinyl alcohol (PVA) incorporating platelet rich plasma (PRP)-encapsulated Alg sulfate (AlgS) microbeads, as a localized sustained release system of GFs, for the articular cartilage regeneration. The results show that synthesized AlgS microbeads support the sustained release of PRP GFs during 14 days, where preserve the bioactivity of them more than the free PRP. Rabbit adipose-derived mesenchymal stem cells in contact with PRP-loaded AlgS beads show more proliferation (2.7 folds) and have obviously higher deposition of collagen type Iota Iota and GAGs than free PRP treated ones. In addition, cells encapsulated into the hydrogel including PRP sustained release system show upregulated expression of collagen type Iota Iota (61 folds), Aggrecan (294 folds) and SOX9 (71.5 folds), as cartilage-critical genes, compared to the direct treatment by PRP. To summarize, the developed hybrid Alg/PVA hydrogel embedding with PRP-encapsulated AlgS microbeads is suggested as a potential in situ formed scaffold for cartilage regeneration.