GSK3 phosphorylates and regulates the Green Revolution protein Rht-B1b to reduce plant height in wheat
GSK3 phosphorylates and regulates the Green Revolution protein Rht-B1b to reduce plant height in wheat
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DOI：
10.1093/plcell/koad090
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发表时间：
2023-03-22
期刊：
影响因子：
11.6
通讯作者：
Sun, Jiaqiang
中科院分区：
文献类型：
--
作者：
Dong, Huixue;Li, Danping;Sun, Jiaqiang
GSK 3磷酸化绿色革命蛋白Rht-B1 b，增强其抑制靶转录因子活性的能力，降低小麦株高。利用稳定的DELLA蛋白Rht-B1 b和Rht-D1 b在小麦绿色革命中提高产量至关重要。然而，其基本机制仍有待澄清。在这里，我们克隆了一个获得的功能等位基因的GSK 3/SHAGGY样激酶编码基因GSK 3的特征小麦矮秆突变体。此外，我们确定GSK 3与绿色革命蛋白Rht-B1 b相互作用并磷酸化，以促进其降低小麦的株高。具体而言，GSK 3的磷酸化可以增强Rht-B1 b的活性和稳定性，使其能够抑制其靶转录因子的活性。综上所述，我们揭示了GSK 3对绿色革命蛋白Rht-B1 b的正调控机制，这可能有助于小麦的绿色革命。
GSK3 phosphorylates the Green Revolution protein Rht-B1b, enhancing its ability to inhibit target transcription factor activity to reduce plant height in wheat.The utilization of stabilized DELLA proteins Rht-B1b and Rht-D1b was crucial for increasing wheat (Triticum aestivum) productivity during the Green Revolution. However, the underlying mechanisms remain to be clarified. Here, we cloned a gain-of-function allele of the GSK3/SHAGGY-like kinase-encoding gene GSK3 by characterizing a dwarf wheat mutant. Furthermore, we determined that GSK3 interacts with and phosphorylates the Green Revolution protein Rht-B1b to promote it to reduce plant height in wheat. Specifically, phosphorylation by GSK3 may enhance the activity and stability of Rht-B1b, allowing it to inhibit the activities of its target transcription factors. Taken together, we reveal a positive regulatory mechanism for the Green Revolution protein Rht-B1b by GSK3, which might have contributed to the Green Revolution in wheat.