Maternal Choline Status, but Not Fetal Genotype, Influences Cord Plasma Choline Metabolite Concentrations.
Maternal Choline Status, but Not Fetal Genotype, Influences Cord Plasma Choline Metabolite Concentrations.
复制标题
DOI：
10.3945/jn.115.211136
复制
发表时间：
2015-05
期刊：
影响因子：
--
通讯作者：
Carly E Visentin;S. Masih;L. Plumptre;O. Malysheva;Daiva E. Nielsen;K. Sohn;A. Ly;A. Lausman;H. Berger;R. Croxford;A. El-Sohemy;M. Caudill;D. O’Connor;Young-In Kim
中科院分区：
文献类型：
--
作者：
Carly E Visentin;S. Masih;L. Plumptre;O. Malysheva;Daiva E. Nielsen;K. Sohn;A. Ly;A. Lausman;H. Berger;R. Croxford;A. El-Sohemy;M. Caudill;D. O’Connor;Young-In Kim
背景：妊娠期胆碱缺乏可导致不良的出生结果，包括神经发育受损和出生缺陷。胆碱和单碳代谢的遗传变异也可能通过改变血浆胆碱浓度来影响出生结果。怀孕期间母亲随意摄入胆碱和胎儿遗传变异对母体和脐带胆碱及其代谢物浓度的影响尚不清楚。目的：本前瞻性研究旨在评估1)母体饮食胆碱摄入量对母体和脐带血浆胆碱及其代谢物浓度的影响，以及2)胎儿遗传多态性对脐带血浆胆碱浓度的影响。方法采用食物频率问卷对368名加拿大孕妇在妊娠早期（0-16周）和晚期（23-37周）的饮食胆碱摄入量进行评估。在母体招募和分娩时以及脐带血中测量血浆游离胆碱及其代谢物浓度。评估了胆碱和单碳代谢的十个胎儿遗传变异与脐带血浆游离胆碱及其代谢物浓度的关系。结果妊娠期间孕妇血浆游离胆碱、二甲基甘氨酸和三甲胺n -氧化物（TMAO）浓度分别升高49%、17%和13% (P < 0.005)，而甜菜碱浓度降低21% （P < 0.005）。脐带血浆游离胆碱、甜菜碱、二甲基甘氨酸和氧化三甲胺浓度分别是分娩时母体相应浓度的3.2、2.0、1.3和0.88倍（均P < 0.005）。孕妇血浆中甜菜碱、二甲基甘氨酸和氧化三甲胺浓度(r(2) = 0.19-0.51；P < 0.0001)，而母体游离胆碱浓度不显著(r(2) = 0.12；P = 0.06)，这些代谢物的脐带血浆浓度的预测因子。母亲的饮食摄入和胎儿的遗传变异都不能预测母体或脐带血浆胆碱及其代谢物的浓度。结论母体胆碱状态影响脐带血浆胆碱代谢物浓度，而非胎儿基因型。该试验在clinicaltrials.gov注册为NCT02244684。
BACKGROUND Choline deficiency during pregnancy can lead to adverse birth outcomes, including impaired neurodevelopment and birth defects. Genetic variants of choline and one-carbon metabolism may also influence birth outcomes by altering plasma choline concentrations. The effects of maternal ad libitum choline intake during pregnancy and fetal genetic variants on maternal and cord concentrations of choline and its metabolites are unknown. OBJECTIVES This prospective study sought to assess the effect of 1) maternal dietary choline intake on maternal and cord plasma concentrations of choline and its metabolites, and 2) fetal genetic polymorphisms on cord plasma concentrations. METHODS The dietary choline intake of 368 pregnant Canadian women was assessed in early (0-16 wk) and late (23-37 wk) pregnancy with the use of a food frequency questionnaire. Plasma concentrations of free choline and its metabolites were measured in maternal samples at recruitment and delivery, and in the cord blood. Ten fetal genetic variants in choline and one-carbon metabolism were assessed for their association with cord plasma concentrations of free choline and its metabolites. RESULTS Mean maternal plasma free choline, dimethylglycine, and trimethylamine N-oxide (TMAO) concentrations increased during pregnancy by 49%, 17%, and 13%, respectively (P < 0.005), whereas betaine concentrations decreased by 21% (P < 0.005). Cord plasma concentrations of free choline, betaine, dimethylglycine, and TMAO were 3.2, 2.0, 1.3, and 0.88 times corresponding maternal concentrations at delivery, respectively (all P < 0.005). Maternal plasma concentrations of betaine, dimethylglycine, and TMAO (r(2) = 0.19-0.51; P < 0.0001) at delivery were moderately strong, whereas maternal concentrations of free choline were not significant (r(2) = 0.12; P = 0.06), predictors of cord plasma concentrations of these metabolites. Neither maternal dietary intake nor fetal genetic variants predicted maternal or cord plasma concentrations of choline and its metabolites. CONCLUSION These data collectively indicate that maternal choline status, but not fetal genotype, influences cord plasma concentrations of choline metabolites. This trial was registered at clinicaltrials.gov as NCT02244684.