A Comparison of the Fine Structure of
A Comparison of the Fine Structure of
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精细结构比较
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通讯作者：
C. M. Carpenter
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作者：
Z. Price;C. M. Carpenter
最早成功地从人的癌肺组织建立细胞系的尝试之一是Cailleau，他在连续培养中繁殖了粘液性腺癌的细胞(10)。她将该细胞系描述为起源于上皮细胞，并将其命名为MAC-21。本报告描述了这些细胞的精细结构，并将它们的微观形态与HeLa细胞进行了比较。HeLa细胞被选为玉米比较研究的原因是因为它的上皮起源，因为已经用光学和电子显微镜对其形态进行了许多研究。然而，大多数此类研究都与病毒感染的病理效应有关(7、21、23、29、30)。只有有限的数量被定向到细胞(9，18)的正常精细结构。除了描述了MAC-21电池的精细结构外，还给出了有关HeLa电池精细结构的进一步数据。很多年了。Mac-21细胞是从R.Cailleau博士那里获得的，随后在我们的实验室进行了增殖。HeLa细胞在含有60%的Difco酵母抽提液、20%的Hyland Sherer维护液、10%的Difco牛肉心脏输液和10%的Hyland小牛血清的培养液中培养。MAC-21细胞在90%的Hyland 199和10%的Hyland小牛血清中培养。分别在生长4天和7天后收获细胞，以下列方式制备电子显微镜：在每个培养管中加入pH为7.4的磷酸缓冲液四氧化三铯，使每个培养管中的最终浓度为1%的OsO4，并在4℃下放置30 mm。随后用3ml.Hamks溶液代替。这些细胞是用橡胶警察刮掉并以每分钟1000转的速度离心机制粒的，从培养管壁上分离出来的。10分钟。颗粒在分级的乙醇中脱水20分钟，并按照LOFT(26)技术包埋在环氧树脂中。切片用Porter-Blum切片机完成，切片用氢氧化铅染色，如Watson(44)所述。本文描述了培养的人肺粘液腺癌细胞(MAC-21)的超微结构，并与HeLa细胞的超微结构进行了比较。…中的差异
One of the first successful attempts to establish a cell line from carcinomatous lung tissue of hu mans was that of Cailleau, who propagated, in continuous culture, cells from a mucoid adenocar cinoma (10). She described this cell line as epi thelial in origin and designated it MAC-21. This report describes the fine structure of these cells and compares their micromorphology with that of HeLa cells. The HeLa cell was selected for corn parative studies because of its epithelial origin and because a number of studies have been made of its morphology with both the optical and electron microscope. The majority of such studies, how ever, have been concerned with pathological effects involving virus infections (7, 21, 23, 29, 30). Only a limited number have been directed toward the normal fine structure of the cell (9, 18). Further data on the fine structure of the HeLa cell are presented, in addition to the description of the fine structure of the MAC-21 cell. of years. MAC-21 cells were obtained from Dr. R. Cailleau and subsequently propagated in our laboratories. The HeLa cells were grown in a culture medium consisting of 60 per cent Difco Yeast Extract Me dium, 20 per cent Hyland Sherer Maintenance So lution, 10 per cent Difco Beef Heart Infusion Broth, and 10 per cent Hyland Calf Serum. The MAC-21 cells were grown in 90 per cent Hyland 199 and Hyland 10 per cent Calf Serum. The cells were harvested after 4 and 7 days of growth, respectively, and prepared for electron microscopy in the following manner: phosphate buffered osmium tetroxide at pH 7.4 was added to give a final concentration of 1 per cent OsO4 in each culture tube and left at 4°C. for 30 mm utes. This was subsequemtly replaced with 3 ml. of Hamks solution. The cells were them detached from the walls of the culture tubes by being scraped with a rubber policeman and pelleted by centrifugatiom at 1,000 r.p.m. for 10 minutes. The pellet was dehydrated for 20-minute periods in graded ethyl alcohols and embedded in epoxy resin following the technic of Luft (26). Sectioning was accomplished with the Porter-Blum microtome The sections were stained with lead hydroxide as described by Watson (44). SUMMARY The fine structure of a cultured mucoid adenocarcinoma cell of human lung (MAC 21) is described and compared with the fine structure of the HeLa cell. The differences in …