Immunomodulatory Effect of Mesenchymal Stem Cells on T Lymphocyte and Cytokine Expression in Nasal Polyps
Immunomodulatory Effect of Mesenchymal Stem Cells on T Lymphocyte and Cytokine Expression in Nasal Polyps
复制标题
DOI：
10.1177/0194599814525751
复制
发表时间：
2014-06-01
影响因子：
3.4
通讯作者：
Roh, Hwan-Jung
中科院分区：
文献类型：
--
作者：
Cho, Kyu-Sup;Kim, Yong-Wan;Roh, Hwan-Jung
目标.脂肪组织来源的干细胞（Adipose tissue-derived stem cells，ASCs）通过诱导T细胞无反应性，对哮喘、过敏性鼻炎等多种炎症性疾病具有免疫调节作用。鼻息肉（NPs）是鼻和鼻旁窦中的慢性炎性疾病，其组织学特征在于炎性细胞如嗜酸性粒细胞或淋巴细胞的浸润。本研究旨在研究ASC是否对嗜酸性粒细胞NPs中的T淋巴细胞和细胞因子表达具有免疫调节作用。基础科学实验研究.设置.大学三级保健设施。主题和方法。NP标本来自20例慢性鼻窦炎和嗜酸性粒细胞NP患者。从15名接受腹腔内手术的受试者的腹部脂肪中分离并培养ASC。从NP组织中分离浸润细胞（1 × 10（6）），并与1 × 10（5）ASC共培养。为探讨ASC是否影响嗜酸性粒细胞NP的浸润T淋巴细胞和细胞因子的表达，检测ASC治疗前后T淋巴细胞亚群和细胞因子的表达。ASC处理显著降低了CD 4（+）和CD 8（+）T细胞的比例。ASC治疗后，Th 2细胞因子（白细胞介素[IL]-4和IL-5）水平显著降低。相反，ASC治疗后Th 1（干扰素-g和IL-2）和调节性细胞因子（转化生长因子-β和IL-10）水平显著升高。ASC在NP的嗜酸性炎症中具有免疫调节作用，其特征在于下调活化的T淋巴细胞和Th 2免疫应答。随着时间的推移，预计这些作用将显著有助于控制嗜酸性粒细胞炎症，并可能有助于嗜酸性粒细胞NP的生长。
Objectives. Adipose tissue-derived stem cells (ASCs) have been reported to have immunomodulatory effects in various inflammatory diseases, including asthma and allergic rhinitis, through the induction of T cell anergy. Nasal polyps (NPs) are a chronic inflammatory disease in the nose and paranasal sinus characterized histologically by the infiltration of inflammatory cells, such as eosinophils or lymphocytes. This study was performed to investigate whether ASCs have immunomodulatory effects on T lymphocyte and cytokine expression in eosinophilic NPs.Study Design. Basic science experimental study.Setting. University tertiary care facility.Subjects and Methods. NP specimens were obtained from 20 patients with chronic rhinosinusitis and eosinophilic NPs. ASCs were isolated and cultured from the abdominal fat of 15 subjects undergoing intra-abdominal surgery. Infiltrating cells (1 x 10(6)) were isolated from NP tissue and co-cultured with 1 x 10(5) ASCs. To determine whether ASCs affect infiltrating T lymphocyte and cytokine expression in eosinophilic NP, T lymphocyte subsets and cytokine expression were analyzed before and after ASC treatment.Results. ASC treatment significantly decreased the proportions of CD4(+) and CD8(+) T cells. After ASC treatment, Th2 cytokine (interleukin [IL]-4 and IL-5) levels decreased significantly. In contrast, levels of Th1 (interferon-g and IL-2) and regulatory cytokines (transforming growth factor-beta and IL-10) increased significantly after ASC treatment.Conclusions. ASCs have immunomodulatory effects in the eosinophilic inflammation of NPs, characterized by down-regulation of activated T lymphocytes and a Th2 immune response. These effects would be expected, over time, to significantly contribute to the control of eosinophilic inflammation and, possibly, growth of eosinophilic NPs.