Innate immune response to intramammary infection with Serratia marcescens and Streptococcus uberis
Innate immune response to intramammary infection with Serratia marcescens and Streptococcus uberis
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DOI：
10.1051/vetres:2004040
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发表时间：
2004-11-01
影响因子：
4.4
通讯作者：
Hope, JC
中科院分区：
文献类型：
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作者：
Bannerman, DD;Paape, MJ;Hope, JC
链球菌和粘质沙雷氏菌分别是革兰氏阳性和革兰氏阴性细菌，可诱导临床型乳腺炎。一旦最初的宿主屏障系统被这些病原体破坏，先天免疫系统就提供了针对这些感染因子的下一级防御。先天性免疫应答的特征在于诱导促炎细胞因子，以及促进宿主识别和消除病原体的其他辅助蛋白的增加。本研究的目的是表征由这两种重要但研究较少的革兰氏阳性和革兰氏阴性微生物引起的临床乳腺炎期间的先天免疫应答。研究了促炎细胞因子反应和先天免疫辅助识别蛋白可溶性CD 14（sCD 14）和脂多糖（LPS）结合蛋白（LBP）水平的变化。产奶量减少、发热反应的诱导和LBP急性期合成增加均是对乳房内感染任一微生物的全身反应的特征。两种细菌的感染同样导致了乳汁中IL-1 β、IL-8、IL-10、IL-12、IFN-γ、TNF-α、sCD 14、LBP和补体成分C5 a水平的升高。然而，这些炎性标志物水平升高的持续时间和/或最大变化对于所测定的几个炎性参数是显著不同的。特别是S.与沙门氏菌感染相比，母乳中IL-1 β、IL-10、IL-12、IFN-γ和C5 a水平持续升高。粘质虫感染总之，这些数据证明了对两种不同乳腺炎病原体的先天免疫应答的可变性。
Streptococcus uberis and Serratia marcescens are Gram-positive and Gram-negative bacteria, respectively, that induce clinical mastitis. Once initial host barrier systems have been breached by these pathogens, the innate immune system provides the next level of defense against these infectious agents. The innate immune response is characterized by the induction of proinflammatory cytokines, as well as increases in other accessory proteins that facilitate host recognition and elimination of the pathogens. The objective of the current study was to characterize the innate immune response during clinical mastitis elicited by these two important, yet less well-studied, Gram-positive and Gram-negative organisms. The pro-inflammatory cytokine response and changes in the levels of the innate immune accessory recognition proteins, soluble CD14 (sCD14) and lipopolysaccharide (LPS)-binding protein (LBP), were studied. Decreased milk output, induction of a febrile response, and increased acute phase synthesis of LBP were all characteristic of the systemic response to intramammary infection with either organism. Infection with either bacteria similarly resulted in increased milk levels of IL-1beta, IL-8, IL-10, IL-12, IFN-gamma, TNF-alpha, sCD14, LBP, and the complement component, C5a. However, the duration of and/or maximal changes in the increased levels of these inflammatory markers were significantly different for several of the inflammatory parameters assayed. In particular, S. uberis infection was characterized by the sustained elevation of higher milk levels of IL-1beta, IL-10, IL-12, IFN-gamma, and C5a, relative to S. marcescens infection. Together, these data demonstrate the variability of the innate immune response to two distinct mastitis pathogens.